AJUBA
Ajuba LIM Protein - Scaffold and Transcriptional Regulator
Gene Information Card
| Symbol | AJUBA |
|---|---|
| Full Name | ajuba LIM protein |
| Gene Type | protein-coding |
| Chromosomal Location | 14q11.2 |
| NCBI Gene ID | 84902 ncbi.nlm.nih.gov/gene/84902 |
| Ensembl ID | ENSG00000129484 |
| UniProt ID | Q96IF1 |
| OMIM ID | 609066 |
| HGNC ID | 20250 |
| Aliases | JUB, FLJ11127, FLJ22965 |
Description
AJUBA encodes a LIM domain-containing protein that functions as a scaffold in cell adhesion complexes and as a transcriptional corepressor. It localizes to cell-cell contacts and the nucleus, modulating signaling pathways including Wnt, Hippo, and TGF-β. AJUBA is involved in cytoskeletal organization, cell migration, and proliferation.
Disease Associations
| Disease category | Pathophysiological mechanism | Genomic evidence |
|---|---|---|
| Hepatocellular carcinoma | AJUBA overexpression promotes tumor growth via YAP/TAZ activation | PMID: 25944712 |
| Colorectal cancer | Loss of AJUBA enhances Wnt/β-catenin signaling and metastasis | PMID: 24703928 |
| Breast cancer | AJUBA downregulation correlates with poor prognosis and EMT | PMID: 27323853 |
| Ovarian cancer | AJUBA mutations impair Hippo signaling and increase cell invasion | PMID: 29056338 |
Expression Profile
Tissue Expression
| Tissue | nTPM | level |
|---|---|---|
| Liver | 8.2 | Medium |
| Colon | 6.5 | Medium |
| Breast | 4.1 | Low |
| Ovary | 5.3 | Medium |
| Lung | 3.8 | Low |
Cell Line Expression
| Cell Line | nTPM | Notes |
|---|---|---|
| HepG2 | 9.1 | Hepatocellular carcinoma cell line |
| HCT116 | 7.4 | Colorectal carcinoma cell line |
| MCF7 | 4.8 | Breast adenocarcinoma cell line |
| OVCAR3 | 5.6 | Ovarian adenocarcinoma cell line |
| A549 | 3.5 | Lung carcinoma cell line |
Data source：Human Protein Atlas（proteinatlas.org）
Mutations & Variants
Hotspot Mutations
| Variant | Type | Frequency | Functional Description |
|---|---|---|---|
| c.487C>T (p.Arg163Trp) | Missense | 0.02% | Loss of LIM domain function |
| c.632_633insA (p.Gln211Glufs*5) | Frameshift | 0.01% | Truncation and loss of nuclear localization |
| c.101G>A (p.Arg34Gln) | Missense | 0.03% | Reduced binding to α-catenin |
Mutation functional classification
Loss of Function (LOF)
Frameshift and nonsense mutations that truncate the protein, disrupting LIM domains and scaffold function.
Gain of Function (GOF)
Not well characterized; some missense variants may enhance YAP/TAZ activity.
Dominant Negative (DN)
Missense mutations in the LIM domain that interfere with wild-type AJUBA binding partners.
View complete mutation data：
Gene Ontology (GO)
| • GO:0005515 - protein binding | • GO:0005634 - nucleus |
| • GO:0005912 - adherens junction | • GO:0005913 - cell-cell adherens junction |
| • GO:0008270 - zinc ion binding | • GO:0019901 - protein kinase binding |
| • GO:0030154 - cell differentiation | • GO:0042802 - identical protein binding |
| • GO:0045892 - negative regulation of transcription | • DNA-templated |
| • GO:0051015 - actin filament binding |
Pathways
• Hippo signaling pathway (KEGG: hsa04390)
• Wnt signaling pathway (KEGG: hsa04310)
• TGF-beta signaling pathway (KEGG: hsa04350)
• Adherens junction (KEGG: hsa04520)
Protein Summary
The Ajuba protein (UniProt Q96IF1) contains three C-terminal LIM domains and a pre-LIM region. It shuttles between the cytoplasm and nucleus, acting as a scaffold at adherens junctions via binding to α-catenin and as a transcriptional corepressor by recruiting histone deacetylases. Ajuba regulates YAP/TAZ activity and is implicated in cancer progression.
Related Services
Related Products
| Product name | Cat.No. | Species | Gene ID | |
|---|---|---|---|---|
| AJUBA Knockout HEK293 Cell Line | EDJ-KQ1361 | Human | 84962 | Details Get a Quote |
| AJUBA Knockout A-549 Cell Line | EDJ-KQ19501 | Human | 84962 | Details Get a Quote |
| AJUBA Knockout HCT 116 Cell Line | EDJ-KQ20849 | Human | 84962 | Details Get a Quote |
| AJUBA Knockout HeLa Cell Line | EDJ-KQ20850 | Human | 84962 | Details Get a Quote |
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