EDITGENE has established a mature gene point mutation editing platform, Bingo™PE7, which is based on the latest Prime Editing editing strategy (PE7). This platform has advanced and mature scheme design and experimental system, which can efficiently and accurately obtain the cell models with random base transition, small fragment knockout or insertion. To resolve the problems in your experimental process, such as inaccurate editing, low editing efficiency, low transfection efficiency, and low efficiency in obtaining homozygotes, to accelerate your research progress.

Technical Principles of Prime Editing

Bingo™ Service Advantages

Efficient editing system

Based on the PE7 gene editing strategy, the editing efficiency and homozygosity probability are greatly improved, which can effectively edit the difficult sites

Efficient pegRNA design algorithm system

The pegRNA design algorithm is exclusively developed with the editing efficiency of up to 100% without off-target events

Enhanced Cas9n-RT editing enzyme system

The optimized Cas9n-RT has stronger editing stability and activity

Efficient transfection system

The unique transfection system that is 10 times more efficient than traditional methods

Efficient single clone sorting technology

The 3D bioprinting technology assists in single clone sorting, the well-plate can fully be seeded with cells in 5 minutes and the cell survival rate is more than three times that of traditional methods. And each clone has an image to ensure it is a single clone

Technical support provided by experienced experts

With over 10 years of gene editing experience for simple and complex editing projects

 

Customization of gene point mutation cell lines

To efficiently obtain the gene point mutation cell lines that meet research requirements

DETAILS

 

Customization of cell lines with precise deletion
or insertion of small fragments

To efficiently obtain the cell lines with deleted or inserted small fragment sequences that meet research requirements

DETAILS

 

Gene therapy drug development based
on PE editing strategy

To design the multiple sets of pegRNAs, evaluate and screen the effective combinations, and obtain the effective gene therapy drugs

DETAILS

 

In-stock gene mutation cell lines

DETAILS

 

Customization of gene point mutation editing vectors

DETAILS

Bingo ™ PE5 is iteratively upgraded by EDITGENE and Bingo ™ PE7 is officially launched with higher editing efficiency.

1) Compared to Bingo ™ PE5, the success rate increased by 4.71 times /span>

Improvement in editing efficiency of PE7 compared to PE5

Targeting site	PE5 editing efficiency	PE7 editing efficiency	The fold increase in editing efficiency of PE7 compared to PE5
LPIN1			2.67
JMID7			4.71
SEC23B			3.83
TMEM41A			2.87
ACE site 1			2.54
PPPIR12B site 1			2.85
PPP1R12B site 2			1.76
TFE3			2.71
NOS3			1.59
TAF6L			1.41
CERS2			1.36
IL4R site 1			1.36
TRIM41			1.23
ODC1			1. 39
KCNI12 site 2			1. 23
KCNJ12 site 1			1. 21
IL4R site 2			1.13

Note: 1 grade<10%, 2 grade20%-30%, 3 grade30%-40%,4 grade50%-60%,5 grade 70%

2) Implementing the effective editing at challenging sites

The editing efficiency of Bingo ™ PE7 is very efficient, which makes it possible to edit challenging sites. We tested multiple difficult sites by using PE7 and PE5 systems, and the results showed that Bingo ™ PE7 can effectively edit these sites. This means that tasks that were previously difficult to edit have become feasible, creating the possibilities for breakthrough research.

Sites where editing failed via PE5, but were successfully edited via PE7

Targeting site	PE5 editing efficiency	PE7 editing efficiency
MMACHC targeting site 1
MMACHC targeting site 2
ACE targeting site 2
ACE targeting site 3

Note: 1 grade<10%, 2 grade20%-30%, 3 grade30%-40%,4 grade50%-60%,5 grade 70%-100%