KPNA2 Knockout HEK293 Cell Line

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KPNA2 Knockout HEK293 Cell Line
Cat.No.:

EDC07572

Species:

Human

Cell Name:

HEK293

Gene:

KPNA2

Gene ID:

3838

Size:

1×10⁶cells

KPNA2 Knockout Cell Line (HEK293) is an exclusive upgraded CRISPR/Cas9 system-mediated gene knockout cell, with the advantages of Optimized Strategy Design, Efficient Cell Transfection, High-Performance Cas9 Protein and Hassle-Free Cell Selection.
Cat.No. EDC07572
Product Name KPNA2 Knockout Cell Line (HEK293)
Cell Line HEK293
Cellosaurus ID CVCL_0045
Cell Line Synonyms Hek293, HEK-293, HEK/293, (HEK)293, HEK 293, HEK,293, 293, 293 HEK, 293 Ad5, Graham 293, Graham-293, Human Embryonic Kidney 293
Gene
NCBI Gene ID
Gene Synonyms IPOA1|PTAC58|QIP2|RCH1|SRP1-alpha|SRP1alpha
Summary
The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Associated Diseases Non-tumor
Morphology Adherent
Passage Ratio 1/5，2days
Complete Culture Medium DMEM + 10% FBS
Freezing Medium 95% Complete culture medium+ 5% DMSO
QC Indels validated by Sanger sequencing; sterility confirmed via microbial testing.
* For research use only. Not intended for use in humans or animals, including clinical, therapeutic, or diagnostic purposes.
LociSTR Info (Sample Cell)
Sample Cell Line: HEK293		STR Info (Cell bank)
Cell Line: HEK293
Allele1Allele2Allele1Allele2
Amelogenin X X
CSF1P0 12 11 12
D2S1338 19 19
D3S1358 15 17 15 17
D5S818 8 8 9
D7S820 11 12 11 12
D8S1179 12 14 12 14
D13S317 12 14 12 14
D16S539 9 13 9 13
D18S51 17 18 17 18
D19S433 15 18 15 18
D21S11 28 30.2 28 30.2
FGA 23 23
Penta D 9 10 9 10
Penta E 7 15 7 15
TH01 7 9.3 7 9.3
TPOX 11 11
vWA 16 19 16 19
D6S1043 11 11
D12S391 19 21 11 15
D2S441 11 15 11 15
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

FAQ

The choice depends on whether you are studying KPNA2 (importin α1, karyopherin α2)'s role as a classical NLS-binding nuclear import receptor or its functions in cancer biology. The Knockout line is the standard tool for asking whether KPNA2 is required for classical nuclear import — KPNA2 partners with importin β1 (KPNB1) to recognize classical monopartite and bipartite NLS sequences on cargo proteins, mediating their nuclear import. Overexpression is useful for studying KPNA2 overexpression in cancer contexts. Important consideration: KPNA family (KPNA1-7) members share substantial substrate scope with cargo-specific preferences — single KPNA2 knockout may show modest phenotypes for some cargoes. Rescue with wild-type or cargo-binding-deficient KPNA2 is the standard specificity control. The knockout is valuable for studying nuclear import biology, KPNA2 overexpression in HCC and other cancers (KPNA2 is a poor prognostic marker), and emerging selective importin inhibitor research.
Primary applications: • Classical nuclear import: nuclear/cytoplasmic fractionation and IF analysis of NLS-containing cargo proteins to characterize KPNA2-dependent import. • HCC and cancer biology: in heterologous hepatocellular contexts, KPNA2 overexpression has been associated with poor HCC prognosis. • KPNA family functional specialization: KPNA1, KPNA3, KPNA4, KPNA5, KPNA6, KPNA7 expression analysis to interpret cargo-specific KPNA2 functions. • Viral protein nuclear import: assessment of viral protein NLS-dependent import in KPNA2-null cells. EDITGENE recommends this model for researchers investigating classical nuclear import biology and KPNA family functional specialization.
Yes. KPNA2 rescue experiments require attention to importin α architecture: • Construct design: use a codon-modified KPNA2 sequence with a small C-terminal tag (FLAG, HA). KPNA2 has N-terminal IBB (importin β-binding) domain and central ARM repeats forming the NLS-binding groove — preserve all elements. • Cargo-binding-deficient rescue: ARM repeat NLS-binding pocket mutations abolish cargo binding. • Importin β-binding-deficient rescue: IBB domain mutations disrupt KPNB1 partnership. • Functional readout: rescue should restore classical NLS-containing cargo nuclear import. HEK293 transduces efficiently with lentivirus and supports stable rescue line generation.
* Research Use Disclaimer: Content is generated from publicly available research data, bioinformatic resources, and computational analyses for research reference only.

Customer Publications

IF=4.5
Frontiers in Microbiology Virology

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