IL4I1 Knockout THP-1 Cell Line

The choice depends on whether you are studying IL4I1 (interleukin 4-induced 1, IL4-induced gene 1)'s role as a secreted L-amino acid oxidase or its functions in human myeloid cell biology and tumor immunosuppression. The Knockout line is the standard tool for asking whether IL4I1 is required for these processes — IL4I1 is a secreted enzyme that oxidatively deaminates L-amino acids (particularly phenylalanine, tryptophan, and tyrosine), generating H₂O₂, ammonia, and α-keto acids; tryptophan-derived indole-3-pyruvate from IL4I1 activates the aryl hydrocarbon receptor (AhR), driving immunosuppression. Overexpression is useful for studying IL4I1 in heterologous expression contexts. For human tumor immunology research, the EDITGENE IL4I1 Knockout in THP-1 is highly relevant — THP-1 is a human acute monocytic leukemia cell line providing a myeloid context for IL4I1 study, where IL4I1 has emerged as a major tumor-associated immunosuppressive factor in tumor-associated macrophages (TAMs) and myeloid-derived suppressor cells. This product complements the parallel Il4i1 Knockout in MC-38 (mouse colon cancer, also available) for cross-species tumor immunology studies. Rescue with wild-type or catalytically-dead IL4I1 enables structure-function studies. The knockout is valuable for studying IL4I1-targeted immuno-oncology drug development — IL4I1 has been compared to IDO1 as a tryptophan-metabolism-based immunosuppression axis.

Primary applications: • Tryptophan metabolism: tryptophan and indole-3-pyruvate (I3P) levels by LC-MS to characterize IL4I1-dependent metabolite generation in human myeloid context. • AhR activation: cellular AhR pathway target gene (CYP1A1, CYP1B1) expression analysis given I3P-mediated AhR activation. • Macrophage immunosuppression: PMA-differentiated THP-1 macrophage studies of IL4I1-driven immunosuppressive phenotypes (T cell suppression assays). • Cross-species comparison: parallel analysis with Il4i1 Knockout in MC-38 (mouse, also available) for cross-species tumor immunology research. EDITGENE recommends this human myeloid model for researchers investigating IL4I1 biology, AhR-mediated immunosuppression, and human IL4I1-targeted immuno-oncology development.

Yes. IL4I1 rescue experiments are well-established for tumor immunology research: • Construct design: use a codon-modified IL4I1 sequence with a small C-terminal tag (FLAG, HA). IL4I1 has N-terminal signal peptide (secreted enzyme), FAD-binding domain, and substrate-binding pocket — preserve all elements. • Catalytically-dead rescue: FAD-binding residue mutations abolish L-amino acid oxidase activity and serve as the standard specificity control. • Secreted enzyme considerations: IL4I1 is secreted — rescue interpretation considers conditioned media transfer or co-culture studies. • Functional readout: rescue should restore cellular indole-3-pyruvate generation and AhR pathway activation. THP-1-specific considerations: • THP-1 is a human acute monocytic leukemia cell line widely used as a monocyte/macrophage model — PMA differentiation generates adherent macrophage-like cells. • Lentiviral transduction efficiency is moderate; suspension and adherent (post-PMA) phenotypes should be characterized separately. • THP-1 retains key monocyte/macrophage markers and TLR responses, making it relevant for myeloid-context Il4i1 studies.