ONE-Step Luciferase Assay System
The One-Step Luciferase Assay System is designed for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture more
Cat.No: EDL0001
Quantity
Applications
• Monitor firefly (Photinus pyralis) luciferase activity in cultured mammalian cells
• High-throughput screening using luciferase reporter cell lines
Notes
• The reagent has been validated in a 96-well format. Other formats will require scaling and optimization by the end-user.
• Luciferase Reagent Buffer must be at ~ room temperature (20- 25°C) before use.
• Luciferase Assay Reagent should be added to cells for at least 5 minutes before measuring luminescence to allow complete cell lysis.
• For maximal light intensity, measure samples within 1 hour of reagent addition.
• Avoid exposing to excessive heat or light during incubation.
• Different cell lines may exhibit variation in lysis ability and/or luminescence signal and may require slight optimization by the end-user.
• Luminescence signal is affected by assay conditions. Results should be compared between samples measured using the same cell type and media/serum combinations.
• To analyze multiple plates, include a common control sample in each plate and normalize the luminescence of each plate to the control contained in the same plate.
• Background luminescence is a characteristic of luminometer performance, therefore, background luminescence must be subtracted from all readings for accuracy.
Shipping Temperature
-20°C to -80°C (gel packs or dry ice)
Storage
Reagents will perform optimally for up to 6 months from date of receipt when the materials are stored as directed. Upon first thaw, store in aliquots at -20°C. The reagent may be subjected to several freeze/thaw cycles with no effect on functionality, but it is recommended that freeze/thaw cycles be avoided whenever possible
