GO:2001280 positive regulation of unsaturated fatty acid biosynthetic process: Mechanism, Genes, Functions and Research Methods
Research-grade guide for scientists and biopharma professionals
Key Takeaways
• GO:2001280 describes any process that activates or increases the frequency, rate or extent of unsaturated fatty acid biosynthetic process, including fatty acid desaturation and polyunsaturated fatty acid biosynthesis.
• Unsaturated fatty acid biosynthesis is controlled by desaturases such as SCD1, and its positive regulation supports cancer cell proliferation and tumorigenesis by inhibiting ferroptosis.
• The oleic acid-PPARγ-FABP4 loop can fuel cholangiocarcinoma colonization in lymph node metastases, linking positive regulation of unsaturated fatty acid biosynthesis to metastatic niche adaptation.
• Gut microbiota and host interactions regulate intramuscular fat deposition in cattle via the alpha-linolenic acid-fatty acid β-oxidation/L-carnitine-MPO axis, showing the term's relevance beyond human disease.
• Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis by regulating gut microbial composition and gut-liver folate and unsaturated fatty acids metabolism.
• Phosphatidylethanolamine regulation of HDL dysfunctionality links poly-unsaturated fatty acids with atherosclerotic cardiovascular diseases, highlighting the pathway's role in lipid transport and cardiovascular risk.
Description
GO:2001280, positive regulation of unsaturated fatty acid biosynthetic process, is a biological process ontology term that captures any molecular event that activates or increases the frequency, rate or extent of unsaturated fatty acid biosynthesis. Unsaturated fatty acids, including monounsaturated and polyunsaturated species, are essential components of membrane phospholipids and precursors for signaling lipids, and their production must be tightly controlled to match cellular demand. The term is therefore central to understanding how cells adjust lipid composition in response to metabolic, immune and oncogenic cues. Researchers study GO:2001280 because dysregulated unsaturated fatty acid biosynthesis contributes to cancer progression, ferroptosis resistance, metabolic liver disease and cardiovascular pathology. For example, overexpression of ZNF488 supports pancreatic cancer cell proliferation and tumorigenesis through inhibition of ferroptosis via regulating SCD1-mediated unsaturated fatty acid metabolism. In cholangiocarcinoma, an oleic acid-PPARγ-FABP4 loop fuels colonization in lymph node metastases microenvironment, directly tying positive regulation of unsaturated fatty acid biosynthesis to metastatic success. Beyond cancer, the term intersects with host-microbe interactions and systemic lipid handling. Rumen microbiota-host interactions regulate intramuscular fat deposition in cattle via the alpha-linolenic acid-fatty acid β-oxidation/L-carnitine-MPO axis, while Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition and gut-liver folate and unsaturated fatty acids metabolism. In cardiovascular biology, phosphatidylethanolamine regulation of HDL dysfunctionality links poly-unsaturated fatty acids with atherosclerotic cardiovascular diseases. These findings make GO:2001280 a high-value target for mechanistic and translational studies.
positive regulation of unsaturated fatty acid biosynthetic process At A Glance
| GO ID | GO:2001280 |
|---|---|
| GO term | positive regulation of unsaturated fatty acid biosynthetic process |
| Ontology | biological_process |
| Synonym | positive regulation of fatty acid desaturation; positive regulation of polyunsaturated fatty acid biosynthesis; positive regulation of unsaturated fatty acid anabolism; positive regulation of unsaturated fatty acid biosynthesis; positive regulation of unsaturated fatty acid formation; positive regulation of unsaturated fatty acid synthesis |
| Major function | Activates or increases the frequency, rate or extent of unsaturated fatty acid biosynthetic process |
| Definition source | QuickGO definition: Any process that activates or increases the frequency, rate or extent of unsaturated fatty acid biosynthetic process. |
| Related processes | Fatty acid desaturation, polyunsaturated fatty acid biosynthesis, lipid metabolism, ferroptosis regulation |
| Disease relevance | Cancer, hepatic steatosis, atherosclerotic cardiovascular disease, metabolic disorders |
| Research methods | CRISPR knockout, point mutation, knock-in, overexpression, CRISPR library screening, bioinformatics |
What Is GO:2001280?
In our own words, GO:2001280 refers to any process that activates or increases the frequency, rate or extent of the biosynthetic process that produces unsaturated fatty acids. This includes positive regulation of fatty acid desaturation, polyunsaturated fatty acid biosynthesis, unsaturated fatty acid anabolism, biosynthesis, formation and synthesis. The term is a biological process and is not itself a gene product; rather, it describes the regulatory inputs that elevate the output of unsaturated fatty acid biosynthetic pathways.
Why Is positive regulation of unsaturated fatty acid biosynthetic process Important in Cell Biology?
GO:2001280 matters because unsaturated fatty acids are not merely structural membrane components; they are signaling molecules and metabolic substrates whose abundance influences cell survival, immune function and systemic lipid homeostasis. Positive regulation of their biosynthesis can determine whether a cancer cell resists ferroptosis, whether a hepatocyte accumulates fat, or whether HDL particles become dysfunctional in atherosclerosis. Understanding the regulators that drive this process therefore provides mechanistic insight into disease and identifies candidate targets for therapeutic intervention.
• Unsaturated fatty acids are essential for membrane fluidity, lipid signaling and energy storage, and their biosynthesis must be positively regulated when demand increases.
• SCD1-mediated unsaturated fatty acid metabolism supports pancreatic cancer cell proliferation and tumorigenesis by inhibiting ferroptosis.
• The oleic acid-PPARγ-FABP4 loop fuels cholangiocarcinoma colonization in lymph node metastases microenvironment, linking this GO term to metastasis.
• Gut microbiota-host interactions regulate intramuscular fat deposition in cattle via the alpha-linolenic acid-fatty acid β-oxidation/L-carnitine-MPO axis.
• Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism.
• Phosphatidylethanolamine regulation of HDL dysfunctionality links poly-unsaturated fatty acids with atherosclerotic cardiovascular diseases.
• Trans fatty acids can interfere with endogenous unsaturated fatty acid metabolism, making positive regulation of desaturation a counter-regulatory node.
• Lipid metabolism drives dietary effects on T cell ferroptosis and immunity, connecting this term to immune cell fate.
• Prostaglandin transport illustrates how unsaturated fatty acid-derived mediators move between compartments, expanding the term's physiological reach.
• CRISPR-based models allow causal testing of candidate regulators of unsaturated fatty acid biosynthesis in disease-relevant cell types.
What Happens During positive regulation of unsaturated fatty acid biosynthetic process?
Initiation of unsaturated fatty acid biosynthesis
In simple terms: The cell starts making unsaturated fats when it senses a need for them.
Positive regulation of unsaturated fatty acid biosynthetic process begins when upstream signals activate the enzymatic machinery that introduces double bonds into fatty acyl chains. This includes desaturases such as SCD1, which convert saturated fatty acids into monounsaturated species, and the broader pathway that generates polyunsaturated fatty acids. The QuickGO definition frames this as any process that activates or increases the frequency, rate or extent of unsaturated fatty acid biosynthetic process, so the initiation step is the regulatory trigger that commits substrate toward desaturation rather than saturation or oxidation.
Desaturation and chain elongation
In simple terms: Enzymes add double bonds and extend the fatty acid chain to make unsaturated products.
Once initiated, the pathway proceeds through desaturation and elongation reactions. SCD1-mediated unsaturated fatty acid metabolism is a well-characterized example in which desaturation produces oleic acid and related monounsaturated species. In cholangiocarcinoma, an oleic acid-PPARγ-FABP4 loop demonstrates how the product of desaturation can feed back to sustain a metastatic microenvironment. The alpha-linolenic acid-fatty acid β-oxidation/L-carnitine-MPO axis in cattle further shows that unsaturated fatty acid biosynthesis is integrated with β-oxidation and carnitine handling, so positive regulation must be considered alongside catabolic pathways.
Incorporation into complex lipids and signaling pools
In simple terms: The newly made unsaturated fats are used to build membranes and signaling molecules.
Unsaturated fatty acids produced by the biosynthetic process are incorporated into phospholipids, triglycerides and cholesteryl esters, and they serve as precursors for signaling lipids. Phosphatidylethanolamine regulation of HDL dysfunctionality links poly-unsaturated fatty acids with atherosclerotic cardiovascular diseases, showing that the fate of unsaturated fatty acids extends to lipoprotein function. Prostaglandin transport illustrates how oxygenated metabolites of unsaturated fatty acids are moved between cells and compartments, which depends on the availability of precursor pools generated by biosynthesis.
Feedback and homeostatic control
In simple terms: The cell monitors unsaturated fat levels and adjusts production to avoid excess or deficiency.
Positive regulation is balanced by feedback mechanisms that sense lipid status. Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism, indicating that host-microbe signals can tune unsaturated fatty acid biosynthesis in the liver. Lipid metabolism also drives dietary effects on T cell ferroptosis and immunity, so the pathway is responsive to nutritional and immune inputs. Trans fatty acids can interfere with endogenous unsaturated fatty acid metabolism, providing an external dietary factor that modulates the pathway.
Key Genes Involved in GO:2001280 positive regulation of unsaturated fatty acid biosynthetic process
The following genes and proteins are experimentally linked to positive regulation of unsaturated fatty acid biosynthetic process or its downstream biology.
| Gene | Major Role | Research Relevance |
|---|---|---|
| SCD1 | Stearoyl-CoA desaturase that introduces a double bond to form monounsaturated fatty acids | SCD1-mediated unsaturated fatty acid metabolism supports pancreatic cancer proliferation and tumorigenesis by inhibiting ferroptosis |
| ZNF488 | Zinc finger protein whose overexpression supports pancreatic cancer cell proliferation | Overexpression of ZNF488 supports pancreatic cancer cell proliferation and tumorigenesis through inhibition of ferroptosis via regulating SCD1-mediated unsaturated fatty acid metabolism |
| PPARG | Nuclear receptor that regulates lipid metabolism and adipocyte biology | Oleic acid-PPARγ-FABP4 loop fuels cholangiocarcinoma colonization in lymph node metastases microenvironment |
| FABP4 | Fatty acid binding protein involved in lipid trafficking | Part of the oleic acid-PPARγ-FABP4 loop in cholangiocarcinoma metastasis |
| ALOX15 | Lipoxygenase that oxygenates polyunsaturated fatty acids | Lipid metabolism drives dietary effects on T cell ferroptosis and immunity |
| GPX4 | Glutathione peroxidase that protects against lipid peroxidation | Ferroptosis regulation is linked to unsaturated fatty acid metabolism in cancer and immunity |
| ACSL4 | Acyl-CoA synthetase that activates long-chain fatty acids | Lipid metabolism drives dietary effects on T cell ferroptosis and immunity |
| LPCAT3 | Lysophosphatidylcholine acyltransferase that incorporates polyunsaturated fatty acids into phospholipids | Lipid metabolism drives dietary effects on T cell ferroptosis and immunity |
| FADS1 | Fatty acid desaturase involved in polyunsaturated fatty acid biosynthesis | Polyunsaturated fatty acid biosynthesis is part of GO:2001280 |
| FADS2 | Fatty acid desaturase involved in polyunsaturated fatty acid biosynthesis | Polyunsaturated fatty acid biosynthesis is part of GO:2001280 |
| ELOVL5 | Elongase that extends polyunsaturated fatty acid chains | Polyunsaturated fatty acid biosynthesis is part of GO:2001280 |
| ELOVL2 | Elongase that extends polyunsaturated fatty acid chains | Polyunsaturated fatty acid biosynthesis is part of GO:2001280 |
| CD36 | Fatty acid translocase that imports fatty acids | Lipid metabolism drives dietary effects on T cell ferroptosis and immunity |
| SREBF1 | Transcription factor that regulates lipogenic gene expression | Positive regulation of unsaturated fatty acid biosynthetic process includes transcriptional control |
| SREBF2 | Transcription factor that regulates cholesterol and lipid biosynthesis | Positive regulation of unsaturated fatty acid biosynthetic process includes transcriptional control |
| NR1H3 | Liver X receptor that regulates lipid metabolism | Positive regulation of unsaturated fatty acid biosynthetic process includes nuclear receptor control |
| PPARA | Nuclear receptor that regulates fatty acid oxidation and lipid metabolism | Alpha-linolenic acid-fatty acid β-oxidation/L-carnitine-MPO axis in cattle |
| CPT1A | Carnitine palmitoyltransferase that controls fatty acid entry into mitochondria | Alpha-linolenic acid-fatty acid β-oxidation/L-carnitine-MPO axis in cattle |
How Is positive regulation of unsaturated fatty acid biosynthetic process Regulated?
Positive regulation of unsaturated fatty acid biosynthetic process is controlled at multiple levels, including transcriptional activation of desaturases and elongases, post-translational modification of enzymes, and substrate availability. The oleic acid-PPARγ-FABP4 loop shows that a lipid product can feed back to sustain a transcriptional program that favors further unsaturated fatty acid production in cholangiocarcinoma. Gut microbiota-host interactions regulate intramuscular fat deposition in cattle via the alpha-linolenic acid-fatty acid β-oxidation/L-carnitine-MPO axis, indicating that microbial and host metabolic signals converge on this pathway. Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism, further demonstrating that the pathway is responsive to the gut-liver axis. Dietary trans fatty acids can interfere with endogenous unsaturated fatty acid metabolism, providing an external regulatory input. Lipid metabolism also drives dietary effects on T cell ferroptosis and immunity, so immune and nutritional status can modulate the pathway.
positive regulation of unsaturated fatty acid biosynthetic process and Human Disease
| Gene | Disease / Biology | Potential Experimental Model |
|---|---|---|
| SCD1 | Pancreatic cancer proliferation and ferroptosis inhibition | Knockout and overexpression in pancreatic cancer cell lines |
| ZNF488 | Pancreatic cancer tumorigenesis | Overexpression and knockout in pancreatic cancer cells |
| PPARG | Cholangiocarcinoma lymph node metastasis | Knock-in and knockout in cholangiocarcinoma models |
| FABP4 | Cholangiocarcinoma colonization | Knockout and overexpression in cholangiocarcinoma cells |
| GPX4 | Ferroptosis and T cell immunity | Point mutation and knockout in T cells |
Cancer and ferroptosis resistance
Positive regulation of unsaturated fatty acid biosynthetic process can promote cancer cell survival by generating unsaturated fatty acids that are incorporated into membrane phospholipids and, when peroxidized, trigger ferroptosis; however, cancer cells often upregulate protective mechanisms. Overexpression of ZNF488 supports pancreatic cancer cell proliferation and tumorigenesis through inhibition of ferroptosis via regulating SCD1-mediated unsaturated fatty acid metabolism. In cholangiocarcinoma, an oleic acid-PPARγ-FABP4 loop fuels colonization in lymph node metastases microenvironment, linking unsaturated fatty acid biosynthesis to metastatic niche adaptation. Lipid metabolism also drives dietary effects on T cell ferroptosis and immunity, which is relevant to immunotherapy and tumor immune evasion.
Metabolic liver disease and steatosis
Hepatic steatosis involves abnormal accumulation of lipids, and unsaturated fatty acid metabolism is a key contributor. Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism. This suggests that positive regulation of unsaturated fatty acid biosynthetic process in the liver can be modulated by microbial interventions and that the pathway is a potential therapeutic node in fatty liver disease.
Cardiovascular disease and lipoprotein dysfunction
Polyunsaturated fatty acids influence lipoprotein function and cardiovascular risk. Regulation of HDL dysfunctionality by phosphatidylethanolamine links poly-unsaturated fatty acids with atherosclerotic cardiovascular diseases. Prostaglandin transport also illustrates how unsaturated fatty acid-derived mediators are moved between compartments, which can affect vascular biology. Therefore, positive regulation of unsaturated fatty acid biosynthetic process may influence the development of atherosclerotic cardiovascular disease through effects on HDL and lipid mediator transport.
Dietary and trans fatty acid effects
Dietary fatty acids can interfere with endogenous unsaturated fatty acid metabolism. Mechanisms of action of trans fatty acids include effects on desaturation and lipid handling, which may oppose positive regulation of unsaturated fatty acid biosynthetic process. This has implications for nutritional strategies and for understanding how diet modifies metabolic disease risk.
From positive regulation of unsaturated fatty acid biosynthetic process-Related Genes to Experimental Models
| Research Question | Suitable Model |
|---|---|
| Is SCD1 causally required for pancreatic cancer cell proliferation? | SCD1 knockout in pancreatic cancer cell lines |
| Does ZNF488 overexpression drive tumorigenesis via unsaturated fatty acid metabolism? | ZNF488 overexpression and knockout in pancreatic cancer cells |
| Does the oleic acid-PPARγ-FABP4 loop promote cholangiocarcinoma metastasis? | PPARG or FABP4 knockout in cholangiocarcinoma cells |
| Can gut microbiota modulate hepatic unsaturated fatty acid metabolism? | Bacteroides thetaiotaomicron colonization in mouse hepatic steatosis models |
| Does phosphatidylethanolamine regulation of HDL link polyunsaturated fatty acids to atherosclerosis? | Knock-in and knockout in cardiovascular cell models |
| How do trans fatty acids affect endogenous unsaturated fatty acid biosynthesis? | Point mutation and overexpression of desaturases in cell models |
How to Study the positive regulation of unsaturated fatty acid biosynthetic process Process
| Method | What It Measures | Typical Application |
|---|---|---|
| CRISPR knockout | Loss-of-function of candidate regulators | Test requirement for SCD1 in cancer cell proliferation |
| CRISPR point mutation | Specific residue function | Dissect catalytic or regulatory sites in desaturases |
| CRISPR knock-in | Tagged or reporter allele expression | Track pathway activity and protein localization |
| Overexpression | Gain-of-function effects | ZNF488 overexpression in pancreatic cancer cells |
| Lipidomics | Unsaturated fatty acid species and complex lipids | Quantify pathway output in steatosis or cancer |
| RNA-seq | Transcriptional changes in desaturases and elongases | Identify positive regulators of the pathway |
| CRISPR library screening | Genome-wide regulators of a phenotype | Discover genes that modulate ferroptosis via unsaturated fatty acids |
| Bioinformatics | Integrated pathway and network analysis | Nominate the alpha-linolenic acid-fatty acid β-oxidation/L-carnitine-MPO axis |
CRISPR knockout and point mutation
CRISPR knockout of candidate regulators such as SCD1 or ZNF488 can test whether they are required for positive regulation of unsaturated fatty acid biosynthetic process and downstream phenotypes like ferroptosis resistance. Point mutations can dissect catalytic residues or regulatory phosphorylation sites in desaturases and transcription factors, providing mechanistic resolution beyond simple loss-of-function.
Overexpression and knock-in
Overexpression of ZNF488 supports pancreatic cancer cell proliferation and tumorigenesis through inhibition of ferroptosis via regulating SCD1-mediated unsaturated fatty acid metabolism, demonstrating the value of gain-of-function models. Knock-in of tagged or mutant alleles can track protein localization and interaction partners in the pathway, while knock-in of reporter cassettes can monitor pathway activity in live cells.
Lipidomics and metabolic profiling
Mass spectrometry-based lipidomics measures the abundance of unsaturated fatty acid species and their incorporation into complex lipids. This is essential to quantify the output of positive regulation of unsaturated fatty acid biosynthetic process and to link it to phenotypes such as hepatic steatosis or HDL dysfunction.
CRISPR library screening and bioinformatics
Genome-wide CRISPR library screening can identify positive regulators of unsaturated fatty acid biosynthesis by selecting for cells that survive ferroptosis inducers or that alter lipid composition. Bioinformatics integration of transcriptomic, lipidomic and CRISPR screen data can nominate pathways such as the alpha-linolenic acid-fatty acid β-oxidation/L-carnitine-MPO axis observed in cattle.
How CRISPR Can Be Used to Study GO:2001280 positive regulation of unsaturated fatty acid biosynthetic process
Knockout
CRISPR knockout is used to delete genes such as SCD1 or ZNF488 to determine whether they are required for positive regulation of unsaturated fatty acid biosynthetic process and associated phenotypes. For example, knockout of SCD1 can reverse the ferroptosis inhibition seen with ZNF488 overexpression in pancreatic cancer cells.
Point Mutation
Point mutation models introduce specific amino acid changes to test the function of catalytic residues, phosphorylation sites or interaction interfaces in desaturases and transcription factors. This provides mechanistic insight that cannot be obtained from complete knockout, especially for enzymes with multiple activities.
Knock-in
Knock-in strategies can insert tags, reporters or disease-associated variants into endogenous loci. This allows tracking of pathway components in real time and testing of whether specific variants alter positive regulation of unsaturated fatty acid biosynthetic process in a physiological context.
Overexpression
Overexpression of candidate genes such as ZNF488 supports pancreatic cancer cell proliferation and tumorigenesis through inhibition of ferroptosis via regulating SCD1-mediated unsaturated fatty acid metabolism, demonstrating how gain-of-function CRISPR models can establish sufficiency. Overexpression can also be used to rescue knockout phenotypes and to test dose-dependent effects on lipid metabolism.
How EDITGENE Supports positive regulation of unsaturated fatty acid biosynthetic process Research
Researchers studying positive regulation of unsaturated fatty acid biosynthetic process-related genes often need to determine whether a candidate gene is causally involved in pathway activation, disease progression or therapeutic response. EDITGENE provides a comprehensive suite of CRISPR-based cell model services to enable such causal experiments with high efficiency and reproducibility.
Contact EDITGENE today to design your custom CRISPR model for positive regulation of unsaturated fatty acid biosynthetic process research.
Frequently Asked Questions About positive regulation of unsaturated fatty acid biosynthetic process
What is GO:2001280?
GO:2001280 is the Gene Ontology term for positive regulation of unsaturated fatty acid biosynthetic process, defined as any process that activates or increases the frequency, rate or extent of unsaturated fatty acid biosynthetic process.
What genes are involved in positive regulation of unsaturated fatty acid biosynthetic process?
Key genes include SCD1, ZNF488, PPARG, FABP4, FADS1, FADS2, ELOVL5 and ELOVL2, with SCD1 and ZNF488 experimentally linked to cancer cell proliferation and ferroptosis inhibition.
How is unsaturated fatty acid biosynthesis regulated?
It is regulated by transcriptional programs, substrate availability and feedback loops such as the oleic acid-PPARγ-FABP4 loop in cholangiocarcinoma, as well as by gut microbiota-host interactions.
Why is positive regulation of unsaturated fatty acid biosynthetic process important in cancer?
It supports cancer cell proliferation and tumorigenesis by inhibiting ferroptosis via SCD1-mediated unsaturated fatty acid metabolism, as shown in pancreatic cancer.
What diseases are linked to unsaturated fatty acid metabolism?
Diseases include pancreatic cancer, cholangiocarcinoma, hepatic steatosis and atherosclerotic cardiovascular diseases.
How can CRISPR be used to study GO:2001280?
CRISPR knockout, point mutation, knock-in and overexpression can test causal roles of genes such as SCD1 and ZNF488 in unsaturated fatty acid biosynthesis and disease phenotypes.
What is the role of SCD1 in unsaturated fatty acid biosynthesis?
SCD1 is a desaturase that introduces double bonds to form monounsaturated fatty acids, and its activity is central to SCD1-mediated unsaturated fatty acid metabolism in cancer.
How does diet affect unsaturated fatty acid biosynthesis?
Dietary trans fatty acids can interfere with endogenous unsaturated fatty acid metabolism, and lipid metabolism drives dietary effects on T cell ferroptosis and immunity.
What model systems are used to study positive regulation of unsaturated fatty acid biosynthetic process?
Common models include pancreatic cancer cell lines, cholangiocarcinoma models, mouse hepatic steatosis models and cardiovascular cell models.
What services does EDITGENE provide for this pathway?
EDITGENE provides knockout, point mutation, knock-in, overexpression cell models, CRISPR library screening and bioinformatics services to study positive regulation of unsaturated fatty acid biosynthetic process.
Conclusion
GO:2001280, positive regulation of unsaturated fatty acid biosynthetic process, is a biologically and clinically significant term that connects lipid metabolism to cancer, metabolic liver disease and cardiovascular pathology. Experimental evidence from pancreatic cancer, cholangiocarcinoma and gut-liver models demonstrates that this pathway is causally involved in disease phenotypes and is amenable to CRISPR-based interrogation. By leveraging knockout, point mutation, knock-in, overexpression and library screening approaches, researchers can dissect the regulators of unsaturated fatty acid biosynthesis and translate these findings into therapeutic strategies. EDITGENE offers the necessary tools and expertise to accelerate such discoveries.
References
- 1. Oteng AB et al.. 2020. Mechanisms of Action of trans Fatty Acids.. Adv Nutr 11(3):697-708 PMID: 31782488
- 2. Wang N et al.. 2026. Lipid metabolism drives dietary effects on T cell ferroptosis and immunity.. Nature 653(8113):200-211 PMID: 41781622
- 3. Xiao Q et al.. 2023. Overexpression of ZNF488 supports pancreatic cancer cell proliferation and tumorigenesis through inhibition of ferroptosis via regulating SCD1-mediated unsaturated fatty acid metabolism.. Biol Direct 18(1):77 PMID: 37986084
- 4. Zhang H et al.. 2024. Oleic acid-PPARγ-FABP4 loop fuels cholangiocarcinoma colonization in lymph node metastases microenvironment.. Hepatology 80(1):69-86 PMID: 38377465
- 5. Zhang WT et al.. 2025. Rumen microbiota-host interactions regulate intramuscular fat deposition in cattle via the alpha-linolenic acid-fatty acid β-oxidation/L-carnitine-MPO axis.. Microbiome 14(1):49 PMID: 41372768
- 6. Li H et al.. 2024. Bacteroides thetaiotaomicron ameliorates mouse hepatic steatosis through regulating gut microbial composition, gut-liver folate and unsaturated fatty acids metabolism.. Gut Microbes 16(1):2304159 PMID: 38277137
- 7. Taradeh M et al.. 2026. Regulation of HDL dysfunctionality by phosphatidylethanolamine links poly-unsaturated fatty acids with atherosclerotic cardiovascular diseases.. Mol Metab 103:102281 PMID: 41248752
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