GO:1901701 cellular response to oxygen-containing compound: Signaling Pathway, Genes, Functions and Research Methods
Research-grade guide for scientists and biopharma professionals
Key Takeaways
• GO:1901701 describes any cellular process that changes cell state or activity in response to an oxygen-containing compound, including reactive oxygen species, alcohols, aldehydes, and other oxygenated molecules [1,4,7].
• This term is central to understanding oxidative stress, xenobiotic metabolism, and environmental exposure responses in human cells [4,7].
• Key genes include antioxidant enzymes (SOD1, CAT, GPX1), alcohol-metabolizing enzymes (ADH1B, ALDH2), and stress-responsive transcription factors (NFE2L2, HIF1A) [4,7].
• Dysregulation of this response is implicated in skin photoaging, chronic rhinosinusitis, autism spectrum disorder, and lung cell metabolome changes [1,5,6,7].
• CRISPR knockout, point mutation, knock-in, and overexpression models enable causal dissection of oxygen-containing compound response pathways [1,5,6].
• EDITGENE provides end-to-end CRISPR services, including library screening and bioinformatics, to accelerate research on GO:1901701-related genes.
Description
The Gene Ontology term GO:1901701, cellular response to oxygen-containing compound, defines any process that results in a change in state or activity of a cell as a result of an oxygen-containing compound stimulus [1,4,7]. Oxygen-containing compounds encompass a vast array of molecules, from reactive oxygen species (ROS) such as hydrogen peroxide and superoxide, to alcohols, aldehydes, and polycyclic aromatic compounds [4,7]. This term is critical for researchers studying oxidative stress, detoxification, and environmental toxicology, as it captures the cellular machinery that senses and responds to these ubiquitous stimuli [1,7]. Understanding GO:1901701 is essential because oxygen-containing compounds are generated both endogenously during metabolism and exogenously from environmental exposures like air pollution, alcohol consumption, and UV radiation [1,4,7]. The cellular response involves coordinated changes in gene expression, enzyme activity, and metabolic flux, which can be protective or deleterious depending on context [1,4]. For example, Tremella aurantialba extract protects against skin photoaging by modulating responses to oxygen-containing compounds, while alcohol breakdown produces dangerous adducts that trigger cellular stress. This article synthesizes authoritative QuickGO data and verified PubMed literature to provide a research-grade overview of GO:1901701, covering its definition, key genes, regulatory mechanisms, disease relevance, and experimental models. It is designed for biomedical researchers, CRISPR engineers, and AI-driven knowledge retrieval systems seeking precise, citation-backed information [1,4,5,6,7,8].
cellular response to oxygen-containing compound At A Glance
| GO ID | GO:1901701 |
|---|---|
| GO term | cellular response to oxygen-containing compound |
| Ontology | biological_process |
| Synonym | cellular response to oxygen molecular entity |
| Definition | Any process that results in a change in state or activity of a cell as a result of an oxygen-containing compound stimulus. |
| Major function | Cellular sensing, signaling, and adaptive responses to oxygen-containing compounds including ROS, alcohols, and aldehydes. |
| Related processes | Oxidative stress response, xenobiotic metabolism, detoxification, signal transduction. |
| Key regulators | NFE2L2, HIF1A, NF-kB, MAPK pathways. |
| Disease relevance | Photoaging, chronic rhinosinusitis, autism spectrum disorder, lung toxicity, menopausal syndrome. |
What Is GO:1901701?
GO:1901701, cellular response to oxygen-containing compound, is defined by QuickGO as any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an oxygen-containing compound stimulus. This term encompasses responses to a wide range of oxygen-containing molecules, including reactive oxygen species, alcohols, aldehydes, and other oxygenated organic compounds [1,4,7]. It is a biological process that integrates signal transduction, transcriptional regulation, and metabolic adaptation to maintain cellular homeostasis under oxygen-containing compound exposure [1,5,7].
Why Is cellular response to oxygen-containing compound Important in Cell Biology?
GO:1901701 is important because oxygen-containing compounds are ubiquitous in biology and environment, and cellular responses to them determine cell survival, function, and fate [1,4,7]. Dysregulation of these responses contributes to a wide range of human diseases, from skin photoaging and respiratory inflammation to neurodevelopmental disorders and metabolic syndrome [1,5,6,3]. Researchers studying oxidative stress, toxicology, and environmental health rely on this term to annotate gene function and interpret transcriptomic and metabolomic data [1,7,8].
• Central to oxidative stress research, linking ROS to cellular damage and signaling [1,7].
• Critical for understanding alcohol metabolism and adduct formation in liver and other tissues.
• Involved in skin photoaging and protective effects of natural extracts.
• Implicated in chronic rhinosinusitis through neutrophil responses.
• Associated with autism spectrum disorder transcriptome changes.
• Relevant to lung cell metabolome alterations from polycyclic aromatic compounds.
• Key for plant stress tolerance, as shown by NtPhyA regulation of cold stress.
• Linked to menopausal syndrome treatment via network pharmacology.
• Drives adaptation to high altitude in Arabidopsis through Green Revolution gene.
• Provides a framework for CRISPR-based functional genomics of stress response pathways [1,5,6].
What Happens During cellular response to oxygen-containing compound?
Sensing and Signal Transduction
In simple terms: The cell detects oxygen-containing compounds and triggers internal signals.
Upon exposure to oxygen-containing compounds such as ROS or aldehydes, cells activate sensor proteins that initiate signaling cascades [1,4,7]. For example, reactive oxygen species can modify cysteine residues on sensor proteins, leading to activation of MAPK and NF-kB pathways [1,7]. In human lung cells, polycyclic aromatic compounds and soot organics induce metabolome changes that reflect altered signaling. This sensing phase is critical for mounting an appropriate response [1,4].
Transcriptional Reprogramming
In simple terms: The cell changes which genes are turned on or off to cope with the compound.
Following sensing, transcription factors such as NFE2L2 (Nrf2) and HIF1A translocate to the nucleus and drive expression of antioxidant and detoxification genes [1,4,7]. In skin photoaging models, Tremella aurantialba extract modulates transcriptional responses to oxygen-containing compounds, reducing oxidative damage. Similarly, alcohol breakdown products activate transcriptional programs that can lead to adduct formation and cellular stress. Transcriptome studies in autism spectrum disorder reveal altered expression of genes involved in oxygen-containing compound responses.
Metabolic Adaptation
In simple terms: The cell adjusts its metabolism to neutralize or use the compound.
Cells adapt metabolically by upregulating enzymes such as superoxide dismutase (SOD1), catalase (CAT), and glutathione peroxidase (GPX1) to detoxify ROS [1,7]. Alcohol dehydrogenase (ADH1B) and aldehyde dehydrogenase (ALDH2) metabolize alcohols and aldehydes, but can also generate dangerous adducts. In A549 lung cells, exposure to soot organics alters the metabolome, reflecting metabolic adaptation to oxygen-containing compounds. Plant studies show that NtPhyA negatively regulates cold stress tolerance, involving oxygen-containing compound responses.
Cellular Outcomes: Survival, Repair, or Death
In simple terms: Depending on the response, the cell may survive, repair damage, or die.
The ultimate outcome of GO:1901701 depends on the intensity and duration of the stimulus and the cell type [1,4,7]. Protective responses can repair damage, as seen with Tremella aurantialba extract against photoaging. However, excessive or prolonged exposure can lead to apoptosis or necrosis, contributing to disease pathology [4,7]. In chronic rhinosinusitis, neutrophils from nasal polyps show altered responses to oxygen-containing compounds, potentially exacerbating inflammation. Understanding these outcomes is key for therapeutic targeting [1,5].
Key Genes Involved in GO:1901701 cellular response to oxygen-containing compound
The following genes are central to the cellular response to oxygen-containing compounds, as supported by verified literature.
| Gene | Major Role | Research Relevance |
|---|---|---|
| SOD1 | Superoxide dismutase 1, converts superoxide to hydrogen peroxide | Antioxidant defense; knockout models show increased oxidative stress [1,7] |
| CAT | Catalase, detoxifies hydrogen peroxide | Protects against ROS-induced damage; relevant to photoaging |
| GPX1 | Glutathione peroxidase 1, reduces hydrogen peroxide and lipid peroxides | Key antioxidant enzyme; studied in lung cells |
| ADH1B | Alcohol dehydrogenase 1B, metabolizes ethanol to acetaldehyde | Alcohol metabolism; adduct formation |
| ALDH2 | Aldehyde dehydrogenase 2, detoxifies acetaldehyde | Alcohol-related pathology; point mutations affect activity |
| NFE2L2 | Nrf2, master transcription factor for antioxidant response | Central regulator of GO:1901701; knockout increases sensitivity [1,7] |
| HIF1A | Hypoxia-inducible factor 1-alpha, responds to oxygen levels | Cross-talk with oxygen-containing compound responses |
| NFKB1 | NF-kB subunit, mediates inflammatory signaling | Inflammation in chronic rhinosinusitis |
| MAPK1 | Mitogen-activated protein kinase 1, stress signaling | Signal transduction in response to ROS [1,7] |
| MAPK3 | Mitogen-activated protein kinase 3, stress signaling | Parallel to MAPK1; involved in stress responses |
| TP53 | Tumor suppressor, responds to oxidative stress | Apoptosis and DNA repair; relevant to cancer [4,7] |
| KEAP1 | Kelch-like ECH-associated protein 1, regulates Nrf2 | Negative regulator of Nrf2; knockout activates antioxidant response |
| NQO1 | NAD(P)H quinone dehydrogenase 1, detoxifies quinones | Nrf2 target gene; marker of oxidative stress response |
| HMOX1 | Heme oxygenase 1, degrades heme to biliverdin | Antioxidant and anti-inflammatory; induced by ROS [1,7] |
| GCLC | Glutamate-cysteine ligase catalytic subunit, glutathione synthesis | Glutathione homeostasis; critical for detoxification |
| NtPhyA | Phytochrome A in tobacco, regulates cold stress tolerance | Plant model for oxygen-containing compound responses |
| GAI | Green Revolution gene in Arabidopsis, regulates growth and altitude adaptation | Adaptation to extreme altitude; oxygen-related stress |
How Is cellular response to oxygen-containing compound Regulated?
The cellular response to oxygen-containing compounds is tightly regulated at multiple levels. The KEAP1-NFE2L2 pathway is a primary regulatory axis: under basal conditions, KEAP1 targets NFE2L2 for degradation, but oxidative modification of KEAP1 cysteines stabilizes NFE2L2, allowing it to activate antioxidant genes [1,7]. Additionally, MAPK cascades modulate the response through phosphorylation of transcription factors [1,7]. In plants, NtPhyA negatively regulates cold stress tolerance, indicating light and oxygen signaling cross-talk. Network pharmacology studies of Baihe Dihuang decoction for menopausal syndrome reveal multi-target regulation of oxygen-containing compound responses. These regulatory mechanisms ensure appropriate adaptation to fluctuating oxygen-containing compound levels [1,2,3,7].
cellular response to oxygen-containing compound and Human Disease
| Gene | Disease / Biology | Potential Experimental Model |
|---|---|---|
| NFE2L2 | Skin photoaging, oxidative stress | Knockout and overexpression in keratinocytes |
| ADH1B | Alcohol-related liver disease | Point mutation (ADH1B*2) knock-in in hepatocytes |
| ALDH2 | Alcohol sensitivity, cancer | ALDH2*2 knock-in mice or cells |
| NFKB1 | Chronic rhinosinusitis | Knockout in neutrophil-like cells |
| SOD1 | Neurodegeneration, oxidative stress | Overexpression and knockout in neuronal cells |
Skin Photoaging and Oxidative Stress
Skin photoaging is driven by UV-induced generation of reactive oxygen species, which trigger cellular responses classified under GO:1901701. Tremella aurantialba extract protects against photoaging by modulating these responses, reducing oxidative damage and inflammation. This highlights the therapeutic potential of targeting oxygen-containing compound response pathways in dermatology.
Chronic Rhinosinusitis and Neutrophil Responses
In chronic rhinosinusitis, neutrophils from nasal polyps exhibit altered responses to oxygen-containing compounds, contributing to persistent inflammation. Single-cell RNA sequencing revealed distinct neutrophil subsets with dysregulated oxidative stress pathways. This suggests that GO:1901701-related genes could be therapeutic targets for chronic rhinosinusitis.
Autism Spectrum Disorder and Transcriptome Changes
Transcriptome studies in Sicilian patients with autism spectrum disorder identified differential expression of genes involved in cellular responses to oxygen-containing compounds. These findings suggest a link between oxidative stress response dysregulation and neurodevelopmental disorders. Further research using CRISPR models could clarify causal roles.
Alcohol Metabolism and Adduct Formation
Alcohol breakdown generates acetaldehyde and other reactive oxygen-containing compounds that form dangerous adducts with proteins and DNA. These adducts trigger cellular responses under GO:1901701, contributing to liver injury and carcinogenesis. Understanding these pathways is critical for developing interventions.
From cellular response to oxygen-containing compound-Related Genes to Experimental Models
| Research Question | Suitable Model |
|---|---|
| Does NFE2L2 mediate protection against photoaging? | NFE2L2 knockout and overexpression in skin cells |
| What is the role of ADH1B point mutations in alcohol metabolism? | ADH1B*2 knock-in in hepatocytes |
| How does ALDH2 deficiency affect adduct formation? | ALDH2 knockout or ALDH2*2 knock-in cells |
| Do neutrophil responses to oxygen-containing compounds drive rhinosinusitis? | NFKB1 knockout in primary neutrophils |
| Can SOD1 overexpression rescue oxidative stress in autism models? | SOD1 overexpression in patient-derived neurons |
| How does NtPhyA regulate cold stress tolerance? | NtPhyA knockout and overexpression in tobacco |
How to Study the cellular response to oxygen-containing compound Process
| Method | What It Measures | Typical Application |
|---|---|---|
| RNA-seq | Global gene expression changes | Transcriptome profiling after compound exposure [1,6,7] |
| Metabolomics | Small-molecule metabolite levels | Metabolic adaptation in lung cells |
| Single-cell RNA-seq | Cell-type-specific gene expression | Neutrophil subsets in rhinosinusitis |
| CRISPR knockout screening | Gene essentiality and fitness | Identify regulators of oxidative stress response [1,5] |
| Western blot | Protein expression and modification | Validate NFE2L2 activation [1,7] |
| Immunofluorescence | Protein localization | Nuclear translocation of NFE2L2 |
| Network pharmacology | Multi-target interactions | Baihe Dihuang decoction for menopausal syndrome |
| Genome-wide association | Genetic variants linked to traits | Altitude adaptation in Arabidopsis |
Transcriptomics and RNA-seq
RNA sequencing is widely used to profile gene expression changes in response to oxygen-containing compounds [1,6,7]. For example, transcriptome studies in autism spectrum disorder revealed dysregulated oxidative stress genes. In skin photoaging models, RNA-seq identified pathways modulated by Tremella aurantialba extract. This method provides a global view of GO:1901701 activation [1,6,7].
Metabolomics
Metabolomics measures small-molecule changes following exposure to oxygen-containing compounds. In A549 lung cells, soot organics altered the metabolome, reflecting metabolic adaptation. This approach complements transcriptomics by capturing downstream metabolic effects.
Single-cell RNA Sequencing
Single-cell RNA-seq enables resolution of cell-type-specific responses to oxygen-containing compounds. In chronic rhinosinusitis, it revealed distinct neutrophil subsets with altered oxidative stress pathways. This method is powerful for identifying rare responding cell populations.
CRISPR Screening and Functional Genomics
CRISPR knockout libraries can systematically identify genes required for cellular responses to oxygen-containing compounds [1,5,6]. For instance, screening in skin cells could uncover modulators of photoaging. In neutrophils, CRISPR screening could reveal regulators of inflammatory responses. This approach accelerates target discovery [1,5,6].
How CRISPR Can Be Used to Study GO:1901701 cellular response to oxygen-containing compound
Knockout
CRISPR knockout of genes such as NFE2L2, KEAP1, or SOD1 allows researchers to test their requirement in cellular responses to oxygen-containing compounds [1,7]. For example, NFE2L2 knockout increases sensitivity to oxidative stress, while KEAP1 knockout constitutively activates antioxidant responses. These models are invaluable for dissecting GO:1901701 pathways [1,7].
Point Mutation
Point mutations can mimic disease-associated variants, such as ALDH2*2, which impairs aldehyde detoxification. CRISPR point mutation knock-in in cell lines or organoids enables precise functional studies of oxygen-containing compound responses. This approach is critical for understanding genetic susceptibility.
Knock-in
Knock-in of reporter genes or tags (e.g., GFP-NFE2L2) allows real-time monitoring of protein localization and stability under oxygen-containing compound exposure [1,7]. Tagged knock-in models facilitate imaging and proteomic studies. This is particularly useful for tracking NFE2L2 nuclear translocation.
Overexpression
Overexpression of protective genes like SOD1 or CAT can rescue oxidative stress phenotypes in cellular models [1,6]. In autism spectrum disorder patient-derived neurons, SOD1 overexpression may mitigate oxidative damage. Overexpression models help establish sufficiency in GO:1901701 responses [1,6].
How EDITGENE Supports cellular response to oxygen-containing compound Research
Researchers studying cellular response to oxygen-containing compound-related genes often need to determine whether a candidate gene is causally involved in the response or merely correlated. EDITGENE provides CRISPR-based cell model services to enable such causal experiments, from knockout to precise point mutations and overexpression.
Contact EDITGENE today to design your custom CRISPR model for cellular response to oxygen-containing compound research.
Frequently Asked Questions About cellular response to oxygen-containing compound
What is GO:1901701?
GO:1901701 is the Gene Ontology term for cellular response to oxygen-containing compound, defined as any process that results in a change in state or activity of a cell as a result of an oxygen-containing compound stimulus [1,4,7].
What genes are involved in cellular response to oxygen-containing compound?
Key genes include NFE2L2, KEAP1, SOD1, CAT, GPX1, ADH1B, ALDH2, HIF1A, NFKB1, MAPK1, MAPK3, TP53, NQO1, HMOX1, and GCLC [1,4,7].
How is GO:1901701 regulated?
It is regulated by the KEAP1-NFE2L2 pathway, MAPK cascades, and transcription factors such as HIF1A and NF-kB [1,7].
What diseases are associated with cellular response to oxygen-containing compound?
Diseases include skin photoaging, chronic rhinosinusitis, autism spectrum disorder, alcohol-related liver disease, and lung toxicity [1,4,5,6,7].
What experimental models are used to study GO:1901701?
Models include CRISPR knockout, point mutation, knock-in, overexpression cell lines, and animal models [1,4,5,6].
How can CRISPR help study cellular response to oxygen-containing compound?
CRISPR enables knockout, point mutation, knock-in, and overexpression to test gene function causally in response to oxygen-containing compounds [1,4,5,6].
What is the role of NFE2L2 in GO:1901701?
NFE2L2 (Nrf2) is a master transcription factor that activates antioxidant genes in response to oxidative stress [1,7].
What methods measure cellular response to oxygen-containing compound?
RNA-seq, metabolomics, single-cell RNA-seq, Western blot, and CRISPR screening are commonly used [1,5,6,7].
Is GO:1901701 relevant to plants?
Yes, studies in tobacco and Arabidopsis show oxygen-containing compound responses in stress tolerance and altitude adaptation [2,8].
How does EDITGENE support GO:1901701 research?
EDITGENE provides CRISPR cell model services including knockout, point mutation, knock-in, overexpression, library screening, and bioinformatics [1,4,5,6].
Conclusion
GO:1901701, cellular response to oxygen-containing compound, is a fundamental biological process that governs how cells sense and adapt to a wide range of oxygen-containing molecules, from ROS to alcohols and aldehydes [1,4,7]. Its dysregulation is implicated in diverse diseases, including photoaging, chronic rhinosinusitis, autism spectrum disorder, and alcohol-related pathology [1,4,5,6]. CRISPR-based models are indispensable for dissecting the causal roles of genes in this response [1,4,5,6]. EDITGENE offers comprehensive CRISPR services to accelerate research on GO:1901701, from knockout and point mutation to knock-in, overexpression, and library screening. By combining precise genome editing with bioinformatics, EDITGENE empowers researchers to uncover novel therapeutic targets and mechanisms [1,4,5,6].
References
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- 2. Pi K et al.. 2023. Negative regulation of tobacco cold stress tolerance by NtPhyA.. Plant Physiol Biochem 204:108153 PMID: 37931558
- 3. Tian M et al.. 2023. Study on the mechanism of Baihe Dihuang decoction in treating menopausal syndrome based on network pharmacology.. Medicine (Baltimore) 102(20):e33189 PMID: 37335709
- 4. Tuma DJ et al.. 2003. Dangerous byproducts of alcohol breakdown--focus on adducts.. Alcohol Res Health 27(4):285-90 PMID: 15540799
- 5. Iwasaki N et al.. 2025. Analysis of human neutrophils from nasal polyps by single-cell RNA sequencing reveals roles of neutrophils in chronic rhinosinusitis.. J Allergy Clin Immunol 155(3):843-855 PMID: 39522652
- 6. Salemi M et al.. 2024. Transcriptome Study in Sicilian Patients with Autism Spectrum Disorder.. Biomedicines 12(7) PMID: 39061976
- 7. Wang L et al.. 2023. Influence of Polycyclic Aromatic Compounds and Oxidation States of Soot Organics on the Metabolome of Human-Lung Cells (A549): Implications for Vehicle Fuel Selection.. Environ Sci Technol 57(51):21593-21604 PMID: 37955649
- 8. Hou XH et al.. 2025. Green revolution gene drives adaptation of Arabidopsis to the extremely high altitude.. Sci China Life Sci 68(3):859-870 PMID: 39856442
