SLC19A1 Knockout Cell Line (HEK293)

The choice depends on whether you are studying SLC19A1 (RFC, reduced folate carrier)'s classical role in folate/antifolate drug uptake or its breakthrough function as the 2'3'-cGAMP importer in innate immune signaling. The Knockout line is the standard tool for both — SLC19A1 is the principal cellular importer of reduced folates, methotrexate, and pemetrexed, AND was identified in 2019 as the major importer of 2'3'-cGAMP for STING-dependent innate immune sensing. Overexpression is useful for testing methotrexate sensitivity or for studying STING agonist pharmacology. For innate immunity research, the EDITGENE SLC19A1 Knockout in HEK293 is a high-value mechanistic tool — extracellular STING agonists (cyclic dinucleotides, ADU-S100, MSA-2 prodrugs, others) often require SLC19A1 for cellular entry. This product complements the parallel SLC19A1 Knockout in HCT 116 (also available); HEK293 is preferred for STING reporter assays and methotrexate biochemistry. Rescue with wild-type or transport-deficient SLC19A1 enables structure-function studies of both folate and cGAMP transport.

Primary applications: • Antifolate drug sensitivity: methotrexate, pemetrexed, and pralatrexate dose-response analysis in SLC19A1-null versus rescued cells. • 2'3'-cGAMP transport: extracellular cGAMP uptake assays measured by STING-dependent IFN-β reporter activation. • STING agonist pharmacology: critical genetic control for testing cyclic dinucleotide STING agonists (ADU-S100, MSA-2 prodrugs, others) that require SLC19A1 for cellular entry. • Folate metabolism: cellular folate pools and one-carbon metabolism analysis in the absence of reduced folate carrier. EDITGENE recommends this model for researchers investigating folate biology, antifolate chemotherapy, and STING-mediated innate immunity.

Yes. SLC19A1 rescue experiments are well-established for both folate and cGAMP transport research: • Construct design: use a codon-modified SLC19A1 sequence with a small C-terminal tag (FLAG, HA). SLC19A1 has 12 transmembrane domains. • Transport-deficient rescue: substrate-binding pocket mutations enable structure-function studies — separate rescue with folate-transport-only versus cGAMP-transport-only mutations (where mapped) can dissect dual functions. • Methotrexate sensitivity rescue: drug sensitivity restoration in SLC19A1-null cells confirms transport function rescue. • STING activation rescue: extracellular cGAMP-induced STING activation restoration confirms cGAMP transport rescue. HEK293 transduces efficiently with lentivirus and is the standard heterologous expression background for SLC19A1 biochemistry.