SLC19A1 Knockout HCT116 Cell Line

The choice depends on whether you are studying SLC19A1 (RFC)'s role in antifolate chemotherapy uptake or its function in innate immune cGAMP transport in colorectal cancer contexts. The Knockout line is the standard tool for both — methotrexate resistance in colorectal cancer is frequently associated with SLC19A1 downregulation, and the knockout serves as a defined resistance background. Overexpression is useful for restoring antifolate drug sensitivity or for studying cGAMP-STING signaling in cancer immunity contexts. For cancer therapy research, the EDITGENE SLC19A1 Knockout in HCT 116 is particularly relevant — it models methotrexate/pemetrexed-resistant colorectal cancer and enables study of STING-mediated tumor immunity in a CRC context. This product complements the parallel SLC19A1 Knockout in HEK293; HCT 116 is preferred for chemotherapy resistance studies and CRC-STING immunity research. Rescue with wild-type or transport-deficient SLC19A1 enables structure-function dissection.

Primary applications: • Methotrexate resistance modeling: drug sensitivity analysis in HCT 116 SLC19A1-null cells as a defined colorectal cancer antifolate resistance background. • STING signaling in CRC: cGAMP-induced STING pathway activation studies in colorectal cancer context. • Tumor immunity studies: assessment of how SLC19A1 loss affects immune cell-tumor interactions through altered cGAMP signaling. • Antifolate prodrug development: PEPT1-targeted methotrexate analogs and SLC19A1-bypassing antifolate compound testing. EDITGENE recommends this HCT 116-based model for cancer-relevant antifolate resistance and tumor STING immunity research; the parallel SLC19A1 Knockout in HEK293 is preferred for biochemical mechanism studies.

Yes. SLC19A1 rescue experiments in HCT 116 are well-suited for cancer-relevant studies: • Construct design: use a codon-modified SLC19A1 sequence with a small C-terminal tag (FLAG, HA). Surface localization must be confirmed before functional studies. • Drug sensitivity rescue: methotrexate/pemetrexed sensitivity restoration in SLC19A1-null HCT 116 confirms transport rescue and serves as a functional positive control. • STING signaling rescue: cGAMP-induced IFN-β reporter activation restoration in cancer cell context. • Functional readout: rescue should restore both folate transport (methotrexate sensitivity) and cGAMP transport (STING activation) — confirming the dual transport function. HCT 116 transduces efficiently with lentivirus and supports stable rescue line generation.