TNFSF11 Knockout HEK293 Cell Line
download
Cat.No.:
EDC07596
Species:
Human
Cell Name:
HEK293
Gene:
TNFSF11
Gene ID:
8600
Size:
1×10⁶cells
TNFSF11 Knockout Cell Line (HEK293) is an exclusive upgraded CRISPR/Cas9 system-mediated gene knockout cell, with the advantages of Optimized Strategy Design, Efficient Cell Transfection, High-Performance Cas9 Protein and Hassle-Free Cell Selection.
| Cat.No. | EDC07596 |
|---|---|
| Product Name | TNFSF11 Knockout Cell Line (HEK 293) |
| Cell Line | HEK293 |
| Cellosaurus ID | CVCL_0045 |
| Cell Line Synonyms | Hek293, HEK-293, HEK/293, (HEK)293, HEK 293, HEK,293, 293, 293 HEK, 293 Ad5, Graham 293, Graham-293, Human Embryonic Kidney 293 |
| Gene | TNFSF11 |
| NCBI Gene ID | |
| Gene Synonyms | CD254|ODF|OPGL|OPTB2|RANKL|TNLG6B|TRANCE|hRANKL2|sOdf |
| Summary |
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dendritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. [provided by RefSeq, Mar 2026]
|
| Associated Diseases | Non-tumor |
| Morphology | Adherent |
| Passage Ratio | 1/5，2days |
| Complete Culture Medium | DMEM + 10% FBS |
| Freezing Medium | 95% Complete culture medium+ 5% DMSO |
| QC | Indels validated by Sanger sequencing; sterility confirmed via microbial testing. |
* For research use only. Not intended for use in humans or animals, including clinical, therapeutic, or diagnostic purposes.
| Loci | STR Info (Sample Cell) Sample Cell Line: HEK293 | STR Info (Cell bank) Cell Line: HEK293 | ||
| Allele1 | Allele2 | Allele1 | Allele2 | |
| Amelogenin | X | X | ||
| CSF1P0 | 12 | 11 | 12 | |
| D2S1338 | 19 | 19 | ||
| D3S1358 | 15 | 17 | 15 | 17 |
| D5S818 | 8 | 8 | 9 | |
| D7S820 | 11 | 12 | 11 | 12 |
| D8S1179 | 12 | 14 | 12 | 14 |
| D13S317 | 12 | 14 | 12 | 14 |
| D16S539 | 9 | 13 | 9 | 13 |
| D18S51 | 17 | 18 | 17 | 18 |
| D19S433 | 15 | 18 | 15 | 18 |
| D21S11 | 28 | 30.2 | 28 | 30.2 |
| FGA | 23 | 23 | ||
| Penta D | 9 | 10 | 9 | 10 |
| Penta E | 7 | 15 | 7 | 15 |
| TH01 | 7 | 9.3 | 7 | 9.3 |
| TPOX | 11 | 11 | ||
| vWA | 16 | 19 | 16 | 19 |
| D6S1043 | 11 | 11 | ||
| D12S391 | 19 | 21 | 11 | 15 |
| D2S441 | 11 | 15 | 11 | 15 |
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
Conclusion: The STR identification of this cell is correct.
FAQ
Which is better for studying TNFSF11 function, TNFSF11 Knockout HEK293 Cell Line or TNFSF11 overexpression HEK293 Cell Line?
The choice depends on whether you are studying RANKL biology or producing recombinant RANKL for osteoclast biology research. Note that TNFSF11 (RANKL) is primarily expressed by osteoblasts, activated T cells, and some tumor cells — its physiological action is on RANK-expressing osteoclast precursors and dendritic cells. The Knockout line in HEK293 is most useful for biochemical and reagent production purposes; HEK293 is the standard recombinant RANKL expression system but is not the physiological producer cell type.
Overexpression is generally more functionally informative than knockout for RANKL research in HEK293. The EDITGENE Knockout line is useful as a negative control for confirming on-target activity of denosumab and similar anti-RANKL therapeutics, and for background-free recombinant RANKL expression. Rescue with membrane-anchored versus soluble RANKL enables isoform-specific studies.
What are the application scenarios for this model?
Primary applications:
• Recombinant RANKL production: HEK293 is widely used for soluble RANKL expression; the knockout ensures background-free recombinant production.
• Anti-RANKL therapeutic specificity: critical genetic control for confirming on-target activity of denosumab and similar therapeutics.
• Membrane versus soluble RANKL studies: comparison of these forms using rescue constructs enables functional dissection of activity differences.
• OPG/RANK/RANKL axis biochemistry: in vitro studies of RANKL interactions with its decoy receptor OPG and signaling receptor RANK.
EDITGENE recommends this model for researchers in bone biology, anti-RANKL therapeutic development, and osteoimmunology biochemistry. Note that physiological osteoclastogenesis requires RANKL-responsive cells (e.g., RAW264.7, primary BMDMs), not HEK293.
Is this TNFSF11 Knockout HEK293 Cell Line compatible with overexpression rescue experiments?
Yes. RANKL rescue experiments require attention to membrane targeting and soluble form generation:
• Construct design: use a codon-modified TNFSF11 sequence with a C-terminal tag (FLAG, HA, His-tag for purification). RANKL is a type II membrane protein cleaved by MMP14 and ADAM10/17 to release the soluble extracellular domain.
• Form-specific rescue: rescue with full-length membrane versus soluble RANKL enables distinguishing their activities — these forms have been reported to have different efficacy in osteoclastogenesis.
• Trimerization validation: RANKL functions as a trimer; proper assembly should be confirmed before functional or biochemical studies.
• Functional readout: rescue should restore secreted RANKL (ELISA) and RANK-binding activity in vitro.
HEK293 is the standard cell line for recombinant RANKL production at yields typically required for osteoclast differentiation reagents and structural studies.
* Research Use Disclaimer: Content is generated from publicly available research data, bioinformatic resources, and computational analyses for research reference only.
Required Accessories
Cell Culture Optimization Series
Related Products
CRISPR Knockout Kit
Flash CRISPR Knockout Kit（Universal Version）Flash CRISPR Knockout Kit（Universal Version）
Flash-Pro CRISPR KO Kit (For Organoids / Stem Cells)Flash-Pro CRISPR KO Kit (For Organoids / Stem Cells)
