SNAP23 Knockout HEK293T Cell Line
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Cat.No.:
EDC07726
Species:
Human
Cell Name:
HEK293T
Gene:
SNAP23
Gene ID:
8773
Size:
1×10⁶cells
SNAP23 Knockout HEK293T Cell Line is an exclusive upgraded CRISPR/Cas9 system-mediated gene knockout cell, with the advantages of Optimized Strategy Design, Efficient Cell Transfection, High-Performotion Cas9 Protein and Hassle-Free Cell Selection.
| Cat.No. | EDC07726 |
|---|---|
| Product Name | SNAP23 Knockout HEK293T Cell Line |
| Species | Human |
| Cell Line | HEK293T |
| Cellosaurus ID | CVCL_0063 |
| Cell Line Synonyms | Hek293T, HEK-293T, HEK 293T, HEK-293-T, HEK 293 T, 293-T, 293 T, 293T, Human Embryonic Kidney 293T, 293tsA1609neo |
| Gene ID | |
| Gene | SNAP23 |
| Summary |
Specificity of vesicular transport is regulated, in part, by the interaction of a vesicle-associated membrane protein termed synaptobrevin/VAMP with a target compartment membrane protein termed syntaxin. These proteins, together with SNAP25 (synaptosome-associated protein of 25 kDa), form a complex which serves as a binding site for the general membrane fusion machinery. Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is present almost exclusively in the brain, suggesting that a ubiquitously expressed homolog of SNAP25 exists to facilitate transport vesicle/target membrane fusion in other tissues. The protein encoded by this gene is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
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| Associated Diseases | Non-tumor |
| Digestion Time | 30 sec~1 min |
| Morphology | Adherent |
| Passage Ratio | 1:5 |
| Complete Culture Medium | DMEM+10% FBS+1% NEAA+1% GlutaMax |
| Freezing Medium | 95% complete culture medium + 5% DMSO |
* For research use only. Not intended for use in humans or animals, including clinical, therapeutic, or diagnostic purposes.
| Loci | STR Info (Sample Cell) Sample Cell Line: HEK293T | STR Info (Cell bank) Cell Line: HEK293T | ||||
| Allele1 | Allele2 | Allele3 | Allele1 | Allele2 | Allele3 | |
| Amelogenin | X | X | ||||
| CSF1PO | 11 | 12 | 11 | 12 | ||
| D2S1338 | 19 | 19 | ||||
| D3S1358 | 15 | 16 | 17 | 15 | 16 | 17 |
| D5S818 | 8 | 9 | 8 | 9 | ||
| D7S820 | 11 | 11 | ||||
| D8S1179 | 11 | 12 | 14 | 12 | 14 | |
| D13S317 | 12 | 14 | 12 | 14 | ||
| D16S539 | 9 | 13 | 9 | 13 | ||
| D18S51 | 17 | 18 | 17 | 18 | ||
| D19S433 | 18 | 18 | ||||
| D21S11 | 28 | 30.2 | 28 | 30.2 | ||
| FGA | 23 | 23 | ||||
| Penta D | 9 | 10 | 9 | 10 | ||
| Penta E | 7 | 15 | 7 | 15 | ||
| TH01 | 7 | 9.3 | 7 | 9.3 | ||
| TPOX | 11 | 11 | ||||
| vWA | 16 | 19 | 16 | 19 | ||
| D6S1043 | 11 | |||||
| D12S391 | 19 | 21 | 19 | 21 | ||
| D2S441 | 11 | 15 | 11 | 15 | ||
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
Conclusion: The STR identification of this cell is correct.
FAQ
Which is better for studying SNAP23 function, SNAP23 Knockout HEK293T Cell Line or SNAP23 overexpression HEK293T Cell Line?
The choice depends on whether you are studying SNAP23's role in non-neuronal SNARE-mediated fusion or its specific functions in regulated exocytosis and recycling pathways. The Knockout line is the standard tool for asking whether SNAP23 is required for membrane fusion events in non-neuronal cells — SNAP23 is the ubiquitously expressed SNAP25 paralog. Overexpression is useful for testing whether elevated SNAP23 is sufficient to drive fusion events or for studying SNAP23 in heterologous fusion reconstitution.
For non-neuronal SNARE research, the EDITGENE SNAP23 Knockout in HEK293T is the standard mechanistic platform — HEK293T's high transfection efficiency facilitates SNARE complex reconstitution studies. This product complements the parallel SNAP23 & STX7 double knockout (also available) for studying combined SNARE function. Rescue with wild-type or SNARE motif-deficient SNAP23 enables structure-function dissection.
What are the application scenarios for this model?
Primary applications:
• Constitutive exocytosis: secretion assays for constitutively trafficked cargo (VSV-G, soluble proteins) in the absence of SNAP23.
• Regulated secretion: where applicable, calcium-triggered exocytosis from HEK293T-relevant secretory pathways.
• SNARE complex reconstitution: in vitro fusion assays with purified SNAP23 and cognate Q-SNARE partners (syntaxin-3, syntaxin-4).
• Paralog studies: comparison with the parallel SNAP23 & STX7 double knockout (also available) for combinatorial SNARE function dissection.
EDITGENE recommends this model for researchers investigating non-neuronal SNARE biology and exocytic membrane fusion mechanisms.
Is this SNAP23 Knockout HEK293T Cell Line compatible with overexpression rescue experiments?
Yes. SNAP23 rescue experiments require attention to SNARE motif function and palmitoylation:
• Construct design: use a codon-modified SNAP23 sequence with a small internal or terminal tag (FLAG, HA). SNAP23 has two SNARE motifs connected by a central palmitoylated linker — small tags should be used to minimize SNARE assembly interference.
• SNARE-deficient rescue: SNARE motif point mutations (e.g., conserved zero-layer residues) abolish four-helix bundle formation and serve as fusion-deficient specificity controls.
• Palmitoylation-deficient rescue: cysteine-to-serine mutations in the palmitoylation linker abolish membrane anchoring and test the importance of membrane association.
• Functional readout: rescue should restore secretion of cargo proteins and exocytic events in HEK293T.
HEK293T transduces with very high efficiency and is the optimal platform for systematic SNARE rescue screening.
* Research Use Disclaimer: Content is generated from publicly available research data, bioinformatic resources, and computational analyses for research reference only.
Required Accessories
Cell Culture Optimization Series
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