SLC52A3 Knockout HCT116 Cell Line

The choice depends on whether you are studying SLC52A3 (RFVT3)'s role in riboflavin (vitamin B2) transport or modeling Brown-Vialetto-Van Laere syndrome. The Knockout line is the standard tool for asking whether RFVT3 is required for cellular riboflavin uptake — RFVT3 is the principal apical riboflavin transporter in intestine. Overexpression is useful for transport kinetics studies and for testing therapeutic riboflavin supplementation strategies. For riboflavin biology research, the EDITGENE SLC52A3 Knockout in HCT 116 is particularly relevant for intestinal riboflavin absorption studies — HCT 116 has an intestinal epithelial origin. SLC52A3 mutations cause Brown-Vialetto-Van Laere syndrome type 2 (motor neuron disease responsive to riboflavin supplementation) — disease variant rescue enables genotype-function studies. Rescue with wild-type or transport-deficient RFVT3 is the standard specificity control.

Primary applications: • Riboflavin uptake: ³H-riboflavin uptake assays to quantify RFVT3-dependent cellular vitamin B2 acquisition. • Disease modeling: rescue with Brown-Vialetto-Van Laere syndrome type 2-associated SLC52A3 mutations for genotype-function studies. • Therapeutic riboflavin response: testing whether high-dose riboflavin can rescue functions in cells expressing specific BVVL mutants. • RFVT paralog studies: SLC52A1 (RFVT1) and SLC52A2 (RFVT2) expression analysis to interpret intestinal riboflavin transport network. EDITGENE recommends this model for researchers investigating riboflavin biology, Brown-Vialetto-Van Laere syndrome, and intestinal vitamin B2 absorption.

Yes. RFVT3 rescue experiments are well-established for riboflavin biology research: • Construct design: use a codon-modified SLC52A3 sequence with a small C-terminal tag (FLAG, HA). The 11 transmembrane SLC52 architecture must be preserved. • BVVL mutation rescue: introduction of patient-derived SLC52A3 mutations enables disease genotype-function correlation in a controlled background. • Therapeutic riboflavin testing: rescue with disease variants combined with high-dose riboflavin treatment models the clinical therapeutic response. • Functional readout: rescue should restore ³H-riboflavin uptake activity. HCT 116 transduces efficiently with lentivirus and is appropriate for intestinal riboflavin absorption studies.