SLC2A11 Knockout Cell Line (HEK293)

The choice depends on whether you are studying SLC2A11 (GLUT11)'s role as a class II fructose-preferring glucose transporter or its tissue-specific functions in skeletal muscle, heart, and kidney. The Knockout line is the standard tool for asking whether GLUT11 is required for cellular hexose transport in non-classical contexts — GLUT11 has substrate selectivity favoring fructose over glucose. Overexpression is useful for testing transport activity in heterologous systems or for studying GLUT11's three reported splice isoforms (GLUT11-A, -B, -C with distinct tissue expression). For GLUT11 research, the EDITGENE Knockout in HEK293 is a mechanistic platform — HEK293 has been extensively used for GLUT family biochemistry. This product complements the parallel SLC2A11 Knockout in HCT 116 (also available); HEK293 is preferred for biochemistry and structure-function studies given its high transfection efficiency and established protocols. Rescue with wild-type, isoform-specific, or transport-deficient GLUT11 enables comprehensive structure-function analysis.

Primary applications: • Fructose vs glucose uptake assays: comparative ³H-fructose and ³H-glucose uptake to characterize GLUT11's reported fructose preference. • Isoform-specific studies: GLUT11-A (muscle/heart/kidney), GLUT11-B, and GLUT11-C isoforms have distinct tissue expression — rescue with specific isoforms enables functional dissection. • GLUT family comparison: parallel analysis with other class II GLUTs (GLUT5, GLUT7, GLUT9) for paralog-specific substrate characterization. • Heterologous expression: biochemical and structure-function studies of GLUT11 in a high-transfection-efficiency background. EDITGENE recommends this HEK293-based model for biochemical and structure-function studies; the parallel SLC2A11 Knockout in HCT 116 is preferred for cancer biology contexts.

Yes. GLUT11 rescue experiments require attention to isoform diversity: • Construct design: use a codon-modified SLC2A11 sequence with a small C-terminal tag (FLAG, HA). GLUT11 has 12 transmembrane domains — preserve canonical GLUT family architecture. • Isoform-specific rescue: GLUT11-A (muscle/heart/kidney), GLUT11-B (kidney/adipose), and GLUT11-C (heart/skeletal muscle) have distinct N-termini — choose isoforms based on the experimental question. • Transport-deficient rescue: substrate-binding pocket mutations enable distinguishing fructose from glucose transport activities. • Functional readout: rescue should restore fructose and glucose uptake activities measured by radiolabel or LC-MS. HEK293 transduces efficiently with lentivirus and supports stable rescue line generation.