RHBDF1 & RHBDF2 Knockout HEK293 Cell Line

The choice depends on whether you are studying combined iRhom function for ADAM17/TACE regulation or distinguishing iRhom-mediated shedding from iRhom-independent processes. The Double Knockout line is uniquely valuable for asking whether ADAM17 maturation and activity are required for these processes — combined iRhom1 + iRhom2 loss essentially eliminates ADAM17 function (ADAM17 fails to exit the ER without iRhom partners), producing an ADAM17 functional null without disrupting the ADAM17 gene itself. Single-iRhom rescue (iRhom1 alone or iRhom2 alone) in the double knockout enables paralog-specific functional studies. For TACE/ADAM17 biology research, the EDITGENE RHBDF1 & RHBDF2 Double Knockout in HEK293 is the gold-standard genetic tool — it produces a functional ADAM17 null and enables clean single-paralog rescue. This product complements the parallel double knockout in A-549. Rescue with iRhom1 alone, iRhom2 alone, or both enables comprehensive paralog dissection — the design for distinguishing iRhom1-versus iRhom2-specific TACE substrate selection. Rescue with iRhom catalytically-dead variants tests whether maturation chaperone activity is sufficient.

Primary applications: • Functional ADAM17 null: combined iRhom1 + iRhom2 loss creates a clean ADAM17 functional null without disrupting the ADAM17 gene — useful for studies separating ADAM17-dependent from ADAM17-independent phenotypes. • Single-iRhom rescue: re-introduction of iRhom1 alone or iRhom2 alone enables paralog-specific TACE substrate selection studies — the gold-standard experimental design. • ADAM17 inhibitor mechanism: critical genetic control for testing TACE inhibitors and antibody-based ADAM17 blockers. • Substrate selectivity: identification of iRhom1-versus iRhom2-preferred substrates through differential rescue analysis. EDITGENE recommends this HEK293-based double knockout as the gold-standard genetic tool for paralog-specific iRhom-ADAM17 research; parallel A-549 double knockout complements for cancer applications.

Yes, and rescue experiments are uniquely powerful in this double knockout background: • Single-iRhom rescue: re-introduction of iRhom1 alone or iRhom2 alone in the double knockout enables paralog-specific TACE substrate selection studies — the gold-standard experimental design. • Combined rescue: simultaneous iRhom1 + iRhom2 re-expression restores ADAM17 maturation capacity and serves as the positive control. • Construct design: codon-modified RHBDF1 or RHBDF2 sequences with small C-terminal tags (FLAG, HA). Both must preserve the rhomboid family pseudoenzyme architecture. • Functional readout: rescue should restore ADAM17 Golgi maturation and substrate shedding; paralog-specific rescue reveals iRhom1-versus iRhom2-preferred substrates. HEK293's very high transfection efficiency enables systematic paralog-specific rescue experiments.