PVRIG Knockout Cell Line (HEK 293)

The choice depends on whether you are studying PVRIG (CD112R)'s role as an emerging immune checkpoint receptor or its interactions with the PVR family (CD155, CD112, TIGIT, CD96, CD226). The Knockout line is the standard tool for asking whether PVRIG is required for inhibitory signaling on T cells and NK cells — PVRIG binds CD112 (PVRL2/nectin-2) and competes with CD226 (DNAM-1), forming an inhibitory pair analogous to TIGIT-CD155. Overexpression is useful for studying PVRIG signaling or for testing receptor-ligand binding. Important consideration: PVRIG is principally expressed on T cells and NK cells — HEK293 is not the physiological context for PVRIG function. The EDITGENE Knockout in HEK293 is most useful for biochemical and antibody specificity validation work. It serves as a critical negative control for anti-PVRIG antibodies in development as cancer immunotherapy agents (COM701/nelistotug and others). For physiological PVRIG immune signaling research, T cell or NK cell models are more appropriate. Rescue with wild-type or signaling-deficient PVRIG enables structure-function studies.

Primary applications: • Anti-PVRIG antibody validation: critical genetic specificity control for anti-PVRIG/CD112R antibodies including COM701 (nelistotug) in clinical development as cancer immunotherapy. • CD112 (PVRL2) binding: in vitro binding assays for PVRIG-CD112 interaction in the absence of endogenous PVRIG. • PVR family receptor studies: PVRIG-CD226 (DNAM-1) competition for CD112 binding, analogous to TIGIT-CD226 competition for CD155. • Heterologous PVRIG expression: structure-function and biochemical studies of PVRIG variants. EDITGENE recommends this model for researchers in cancer immunotherapy antibody development and PVR family receptor biochemistry. Physiological PVRIG inhibitory signaling research requires T cell or NK cell models.

Yes. PVRIG rescue experiments are well-established for immune checkpoint research: • Construct design: use a codon-modified PVRIG sequence with a small intracellular C-terminal tag (FLAG, HA). PVRIG is a type I membrane protein with an N-terminal Ig-V domain (CD112 binding) and intracellular ITIM/ITT-like motifs — preserve all elements. • Surface localization validation: confirm plasma membrane localization by cell surface staining before functional assays. • Signaling-deficient rescue: cytoplasmic tail ITIM/ITT-like motif mutations enable separation of ligand binding from intracellular inhibitory signaling. • Functional readout: rescue should restore CD112 binding (flow cytometry) and antibody recognition for antibody specificity validation. HEK293 transduces efficiently with lentivirus and supports stable rescue line generation.