NPR3 Knockout Cell Line (HeLa)

The choice depends on whether you are studying NPR3 (NPR-C)'s role as the natriuretic peptide clearance receptor or its emerging functions in signaling distinct from clearance. The Knockout line is the standard tool for asking whether NPR-C is required for clearing ANP, BNP, and CNP from circulation — NPR-C lacks guanylyl cyclase activity and primarily internalizes/degrades natriuretic peptides, regulating their bioavailability. Overexpression is useful for studying NPR-C signaling functions or for testing NPR-C-selective ligand specificity. For natriuretic peptide research, the EDITGENE NPR3 Knockout in HeLa enables study of clearance-deficient natriuretic peptide signaling. This product complements the parallel NPR1 and NPR2 Knockouts in HeLa (also available); the NPR3 KO is unique in that loss enhances ANP/BNP/CNP signaling by reducing clearance. Rescue with wild-type or clearance-deficient NPR-C is the standard specificity control.

Primary applications: • Natriuretic peptide clearance: ¹²⁵I-ANP, ¹²⁵I-BNP, or ¹²⁵I-CNP cellular uptake assays in NPR3-null cells to quantify NPR-C-dependent clearance. • Enhanced NP signaling: assessment of ANP/BNP/CNP-induced cGMP and downstream effects in the NPR3-null context, where clearance is impaired. • NPR-C signaling studies: Gi-coupled signaling readouts given NPR-C's reported signaling functions distinct from clearance. • Pharmaceutical NP analog studies: testing of clearance-resistant NP analogs (e.g., carperitide, BD-CNP) in NPR3-null cells. EDITGENE recommends this model for researchers investigating natriuretic peptide clearance and NPR-C signaling biology.

Yes. NPR-C rescue experiments require attention to receptor topology: • Construct design: use a codon-modified NPR3 sequence with a small intracellular C-terminal tag (FLAG, HA). NPR-C has extracellular natriuretic peptide-binding domain, single transmembrane span, and short intracellular C-terminus — NPR-C lacks guanylyl cyclase activity. • Internalization-deficient rescue: cytoplasmic tail mutations may affect internalization/clearance without affecting binding. • Functional readout: rescue should restore natriuretic peptide clearance (¹²⁵I-NP uptake) and reduce ambient ANP/BNP/CNP levels. HeLa transduces efficiently with lentivirus and supports stable rescue line generation.