MAS1 Knockout Cell Line (A549)

The choice depends on whether you are studying MAS1 (MAS receptor)'s role as the angiotensin (1-7) [Ang(1-7)] receptor or its functions in the protective renin-angiotensin system (RAS) axis. The Knockout line is the standard tool for asking whether MAS1 is required for these processes — MAS1 is a Gq-coupled GPCR activated by Ang(1-7), counterbalancing the classical AT1R-mediated vasoconstriction/pro-fibrotic signaling. The ACE2-Ang(1-7)-MAS axis is considered the protective arm of the renin-angiotensin system. Overexpression is useful for studying MAS1 in heterologous expression contexts. For cardiovascular and SARS-CoV-2 research, the EDITGENE MAS1 Knockout in A-549 is highly relevant — A-549 expresses ACE2 (the SARS-CoV-2 entry receptor that also generates Ang(1-7)), and the MAS1 axis has emerged as relevant to COVID-19 pathophysiology and lung biology. Rescue with wild-type or signaling-deficient MAS1 enables structure-function studies. The knockout is valuable for studying MAS1 agonists (AVE0991 and related compounds) in cardiovascular and pulmonary protection research.

Primary applications: • Ang(1-7) signaling: intracellular calcium mobilization, ERK activation, and NO production following Ang(1-7) stimulation to characterize MAS1-dependent signaling. • ACE2-Ang(1-7)-MAS axis: integrated studies of the protective RAS arm in lung biology and inflammation contexts. • SARS-CoV-2 pathophysiology: studies of MAS1 axis dysregulation following SARS-CoV-2 infection given ACE2's dual roles as viral receptor and Ang(1-7) generator. • MAS1 agonist specificity: critical genetic control for AVE0991 and other MAS1-targeting compounds in cardiovascular and pulmonary protection research. EDITGENE recommends this model for researchers investigating the protective RAS axis, MAS1 signaling, and ACE2-Ang(1-7)-MAS biology relevant to cardiovascular and pulmonary disease.

Yes. MAS1 rescue experiments require attention to GPCR topology: • Construct design: use a codon-modified MAS1 sequence with a small intracellular C-terminal tag (FLAG, HA). MAS1 is a seven-transmembrane GPCR — preserve extracellular Ang(1-7)-binding region. • Signaling-deficient rescue: DRY motif mutations disrupt G-protein coupling. • Functional readout: rescue should restore Ang(1-7)-induced calcium mobilization, ERK activation, and NO production. A-549 transduces efficiently with lentivirus and supports stable rescue line generation.