IGFLR1 Knockout Cell Line (HEK293)

The choice depends on the experimental question. IGFLR1 (IGF-like family receptor 1, TMEM149) is an emerging T cell costimulatory molecule with limited functional characterization. The Knockout line is appropriate for asking whether IGFLR1 is required for predicted activities — IGFLR1 has been characterized as a costimulatory molecule expressed on activated T cells and Treg cells, with TNFR-family-like structural features and emerging roles in T cell activation and tumor immunology. Overexpression is useful for studying IGFLR1 in heterologous expression contexts. For T cell costimulation research, the EDITGENE IGFLR1 Knockout in HEK293 provides a clean genetic background for IGFLR1 structure-function studies. Rescue with wild-type IGFLR1 is the standard specificity control. The knockout is valuable for studying IGFLR1-mediated T cell costimulation and emerging IGFLR1-targeted immunotherapy approaches — IGFLR1 is a less-characterized but emerging cancer immunotherapy target distinct from canonical checkpoint molecules.

Primary applications: • T cell costimulation studies: in heterologous T cell-relevant contexts, characterization of IGFLR1-mediated T cell activation. • Treg biology: in heterologous Treg-relevant contexts, IGFLR1's role in regulatory T cell function. • Surface expression characterization: IGFLR1 plasma membrane expression and ligand identification studies. • Discovery proteomics: interactome analysis in IGFLR1-null versus rescued cells. EDITGENE recommends this model for researchers investigating IGFLR1 biology and emerging T cell costimulation pathways distinct from canonical checkpoint molecules.

Yes. IGFLR1 rescue experiments require attention to membrane topology: • Construct design: use a codon-modified IGFLR1 sequence with a small intracellular C-terminal tag (FLAG, HA). IGFLR1 has the TNFR-family-like architecture (extracellular ligand-binding region, single transmembrane span, intracellular tail) — preserve all elements. • Surface localization validation: confirm plasma membrane localization by cell surface staining before functional assays. • Discovery-oriented rescue: parallel wild-type rescue during phenotypic characterization distinguishes IGFLR1-dependent phenotypes. • Functional readout: rescue should restore phenotypes identified during knockout characterization. HEK293 transduces efficiently with lentivirus and supports stable rescue line generation.