Product Details
| Cat.No. | EDC07558 | Product Name | FOXN3 Knockout A549 Cell Line |
|---|---|---|---|
| Cell Line | A-549 | Cellosaurus ID | CVCL_0023 |
| Cell Line Synonyms | A 549, A549, NCI-A549, A549/ATCC, A549 ATCC, A549ATCC, hA549 | Gene | |
| NCBI Gene ID | Gene Synonyms | C14orf116|CHES1|PRO1635 | |
| Summary |
This gene is a member of the forkhead/winged helix transcription factor family. Checkpoints are eukaryotic DNA damage-inducible cell cycle arrests at G1 and G2. Checkpoint suppressor 1 suppresses multiple yeast checkpoint mutations including mec1, rad9, rad53 and dun1 by activating a MEC1-independent checkpoint pathway. Alternative splicing is observed at the locus, resulting in distinct isoforms. [provided by RefSeq, Jul 2008]
|
Associated Diseases | Non-Small Cell Lung Carcinoma |
| Morphology | Adherent | Passage Ratio | 1/5-1/4 ,2days |
| Complete Culture Medium | F-12K + 10% FBS | Freezing Medium | 95% Complete culture medium + 5% DMSO |
| QC | Indels validated by Sanger sequencing; sterility confirmed via microbial testing. |
* For research use only. Not intended for use in humans or animals, including clinical, therapeutic, or diagnostic purposes.
STR Profile
| Loci | STR Info (Sample Cell) Sample Cell Line: A-549 | STR Info (Cell bank) Cell Line: A-549 | ||
| Allele1 | Allele2 | Allele1 | Allele2 | |
| Amelogenin | X | Y | X | Y |
| CSF1PO | 10 | 12 | 10 | 12 |
| D2S1338 | 24 | 24 | ||
| D3S1358 | 16 | 16 | ||
| D5S818 | 11 | 11 | ||
| D7S820 | 8 | 11 | 8 | 11 |
| D8S1179 | 13 | 14 | 13 | 14 |
| D13S317 | 11 | 11 | ||
| D16S539 | 11 | 12 | 11 | 12 |
| D18S51 | 14 | 17 | 14 | 17 |
| D19S433 | 13 | 13 | ||
| D21S11 | 29 | 29 | ||
| FGA | 23 | 23 | ||
| Penta D | 9 | 9 | ||
| Penta E | 7 | 11 | 7 | 11 |
| TH01 | 8 | 9.3 | 8 | 9.3 |
| TPOX | 8 | 11 | 8 | 11 |
| vWA | 14 | 14 | ||
| D6S1043 | 11 | 13 | ||
| D12S391 | 18 | 18 | ||
| D2S441 | 10 | 13 | 10 | 13 |
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
Conclusion: The STR identification of this cell is correct.
FAQ
Which is better for studying FOXN3 function, FOXN3 Knockout A-549 Cell Line or FOXN3 overexpression A-549 Cell Line?
The choice depends on whether you are studying FOXN3's role in a complementary lung cancer background for cross-validation of FOXN3-dependent phenotypes. The Knockout line is the standard tool for asking whether FOXN3 is required for these processes in NSCLC context. Overexpression is useful for studying FOXN3 gain-of-function effects.
For lung cancer research, the EDITGENE FOXN3 Knockout in A-549 enables study of FOXN3 in NSCLC context. This product complements the parallel FOXN3 Knockout in Hep-G2 (also available); cross-background validation strengthens characterization of FOXN3-dependent phenotypes. Rescue with wild-type FOXN3 is the standard specificity control. The knockout is valuable for studying FOXN3 in lung cancer biology.
Is this FOXN3 Knockout A-549 Cell Line compatible with overexpression rescue experiments?
Yes. FOXN3 rescue experiments in A-549 are well-suited for cross-validation:
• Construct design: same considerations as FOXN3/Hep-G2 rescue — preserve forkhead DNA-binding domain.
• Cross-background comparison: rescue in A-549 alongside Hep-G2 enables confirmation of context-independent FOXN3 functions.
• Functional readout: rescue should restore FOXN3-dependent phenotypes identified during knockout characterization.
A-549 transduces efficiently with lentivirus and supports stable rescue line generation.
* Research Use Disclaimer: Content is generated from publicly available research data, bioinformatic resources, and computational analyses for research reference only.
