DTWD1 Knockout AML12 Cell Line

The choice depends on whether you are studying Dtwd1 (DTW domain containing 1)'s role as a tRNA aminocarboxypropyltransferase in murine hepatocyte context (see DTWD1/HEK293 entry for nomenclature clarification — DTWD1 is an acp³U modifier, not a pseudouridine synthase). The Knockout line is the standard tool for asking whether Dtwd1 is required for acp³U modification at tRNA position 20 in murine hepatocytes — Dtwd1 catalyzes the SAM-dependent acp³U modification, contributing to tRNA structural stability and translation fidelity. Overexpression is useful for studying Dtwd1 in heterologous expression contexts. For murine hepatic tRNA modification research, the EDITGENE Dtwd1 Knockout in AML12 is uniquely valuable — AML12 is a non-tumorigenic murine hepatocyte cell line, providing a physiologically relevant context for liver tRNA biology. This product complements the parallel DTWD1 Knockout in HEK293 (human, also available) for cross-species studies. Rescue with wild-type Dtwd1 is the standard specificity control. The knockout is valuable for studying tRNA modification biology in murine hepatic context.

Primary applications: • Murine hepatic tRNA modification: acp³U levels in murine liver-context tRNAs. • Cross-species comparison: parallel analysis with DTWD1 Knockout in HEK293 (human, also available) for cross-species acp³U biology. • Hepatic translation biology: ribosome profiling and translation analysis in hepatocyte context. • Liver-specific phenotypes: assessment of hepatic gene expression and metabolism given AML12's hepatocyte-like nature. EDITGENE recommends this AML12-based model for researchers investigating hepatic tRNA modification biology in murine context.

Yes. Dtwd1 rescue experiments in AML12 require attention to species and acp³U transferase architecture: • Construct design: use a codon-modified murine Dtwd1 sequence with a small C-terminal tag (FLAG, HA). Preserve DTW domain catalytic architecture. • Cross-species considerations: human DTWD1 can rescue murine Dtwd1 given evolutionary conservation, but species-matched rescue is preferred. • Functional readout: rescue should restore tRNA acp³U levels in hepatic tRNAs. AML12-specific considerations: • AML12 is a non-tumorigenic immortalized murine hepatocyte cell line (TGF-α transgenic CD1 mouse origin) retaining hepatocyte differentiation features. • Lentiviral transduction is supported but may require optimization. • Specialized culture conditions (DMEM/F12 with ITS-dexamethasone supplement) are required.