CTH Knockout Cell Line (A549)

The choice depends on whether you are studying CTH (cystathionine γ-lyase, CSE)'s role in transsulfuration and H₂S biosynthesis or modeling its functions in lung cancer and cysteine metabolism. The Knockout line is the standard tool for asking whether CTH is required for these processes — CTH catalyzes the second step of transsulfuration (cystathionine → cysteine + α-ketobutyrate + NH₃), generating cysteine from methionine-derived homocysteine; CTH is also one of three enzymes (with CBS and 3-MST) that generates H₂S, an endogenous gaseous signaling molecule with cardiovascular and anti-inflammatory effects. Overexpression is useful for studying CTH gain-of-function effects. For lung cancer and metabolic research, the EDITGENE CTH Knockout in A-549 is highly relevant — A-549 is an NSCLC cell line, and CTH-derived cysteine/H₂S has been implicated in NSCLC biology including glutathione synthesis and redox balance. Rescue with wild-type or catalytically-dead CTH enables structure-function studies. CTH biallelic loss causes cystathioninuria (autosomal recessive metabolic disorder). The knockout is valuable for studying transsulfuration biology, H₂S signaling, ferroptosis (cysteine availability for GSH synthesis), and emerging CTH-targeted approaches.

Primary applications: • Transsulfuration biology: cysteine, glutathione (GSH) levels and homocysteine accumulation analysis in CTH-null cells. • H₂S production: cellular H₂S quantification by fluorescent probes (SF-7AM, AzMC) given CTH's role as one of three H₂S-generating enzymes. • Ferroptosis sensitivity: GPX4 activity and lipid peroxidation analysis given CTH's contribution to cysteine availability for GSH-GPX4 axis. • Lung cancer biology: NSCLC proliferation and chemotherapy sensitivity in A-549 context. EDITGENE recommends this lung cancer model for researchers investigating transsulfuration biology, H₂S signaling, ferroptosis defense, and CTH-targeted approaches.

Yes. CTH rescue experiments are well-established for transsulfuration research: • Construct design: use a codon-modified CTH sequence with a small C-terminal tag (FLAG, HA). CTH has the canonical PLP (pyridoxal 5'-phosphate)-dependent enzyme architecture — preserve all elements. • Catalytically-dead rescue: PLP-binding lysine mutations abolish γ-lyase activity and serve as the standard specificity control. • Functional readout: rescue should restore cysteine production from cystathionine and H₂S generation activity. A-549 transduces efficiently with lentivirus and supports stable rescue line generation.