Product Details
| Cat.No. | EDC07987 | Product Name | BRD1 Knockout HAP1 Cell Line |
|---|---|---|---|
| Species | Human | Cell Line | HAP1 |
| Cellosaurus ID | CVCL_0F62 | Cell Line Synonyms | Highly Aggressively Proliferating Immortalized |
| NCBI Gene ID | Gene | ||
| Gene Synonyms | BRL|BRPF2 | Summary |
This gene encodes a bromodomain-containing protein that localizes to the nucleus and can interact with DNA and histone tails. The encoded protein is a component of the MOZ/MORF acetyltransferase complex and can stimulate acetylation of histones H3 and H4, thereby potentially playing a role in gene activation. Variation in this gene is associated with schizophrenia and bipolar disorder in some study populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2017]
|
| Digestion Time | 2 min | Morphology | Adherent |
| Passage Ratio | 1:8~1:10 | Complete Culture Medium | IMDM+10%FBS |
| Freezing Medium | 90%FBS+10%DMSO |
* For research use only. Not intended for use in humans or animals, including clinical, therapeutic, or diagnostic purposes.
FAQ
Which is better for studying BRD1 function, BRD1 Knockout HAP1 Cell Line or BRD1 overexpression HAP1 Cell Line?
The choice depends on whether you are studying BRD1's role as the principal histone H4 acetyltransferase complex scaffold or modeling its emerging functions in hematologic biology. The Knockout line is the standard tool for asking whether BRD1 is required for these processes — BRD1 (BRPF2) is a bromodomain-containing protein that scaffolds the BRD1-HBO1 (KAT7/MYST2)-MEAF6-ING5 histone acetyltransferase complex, deposing the H4K12ac mark on chromatin; BRD1 is critical for proper hematopoiesis and has reported emerging functions in psychiatric disease GWAS. Overexpression is useful for studying BRD1 in heterologous expression contexts.
For histone acetyltransferase research, the EDITGENE BRD1 Knockout in HAP1 enables study of HBO1 complex biology. Other bromodomain family member expression analysis aids interpretation. Rescue with wild-type or bromodomain-mutant BRD1 enables structure-function studies. The knockout is valuable for studying H4K12ac deposition, HBO1 complex biology, and emerging BRD1/HBO1-targeted approaches (HBO1 inhibitors WM-1119 in preclinical development for AML).
Is this BRD1 Knockout HAP1 Cell Line compatible with overexpression rescue experiments?
Yes. BRD1 rescue experiments require attention to HBO1 complex assembly:
• Construct design: use a codon-modified BRD1 sequence with a small C-terminal tag (FLAG, HA). BRD1 has PWWP, bromodomain, and zinc finger domains — preserve all elements.
• Bromodomain-mutant rescue: BRD acetyl-mark recognition pocket mutations.
• HBO1 complex assembly: rescue interpretation considers HBO1 (KAT7/MYST2), MEAF6, ING5 expression.
• Functional readout: rescue should restore H4K12ac deposition.
HAP1-specific considerations:
• Diploidization: HAP1 cells gradually diploidize during extended culture — confirm ploidy by flow cytometry at the time of phenotypic assay.
• Integration site sensitivity: position effects on transgene expression are more pronounced in near-haploid backgrounds; generating multiple independent rescue clones is strongly recommended.
• Transduction efficiency: HAP1 transduces with lentivirus at moderate efficiency — increase MOI compared to standard immortalized lines.
* Research Use Disclaimer: Content is generated from publicly available research data, bioinformatic resources, and computational analyses for research reference only.
