ALOX5AP Knockout Cell Line (HEK293)

The choice depends on whether you are studying ALOX5AP (5-lipoxygenase-activating protein, FLAP)'s role as the essential co-factor for ALOX5/5-lipoxygenase activation or modeling leukotriene biology. The Knockout line is the standard tool for asking whether FLAP is required for these processes — FLAP is a membrane-embedded protein at the nuclear envelope that scaffolds arachidonic acid delivery to ALOX5/5-LOX for leukotriene A4 (LTA4) synthesis; LTA4 is then converted to LTB4 (potent neutrophil chemoattractant) or cysteinyl leukotrienes (LTC4, LTD4, LTE4 — bronchoconstrictors in asthma). Overexpression is useful for studying FLAP gain-of-function effects. For leukotriene biology research, the EDITGENE ALOX5AP/FLAP Knockout in HEK293 is uniquely valuable — FLAP KO completely abolishes leukotriene synthesis. This product complements the parallel ALOX5 Knockout in HEK293 (also available) for systematic 5-LOX pathway dissection. Rescue with wild-type FLAP is the standard specificity control. The knockout is a critical specificity tool for ⭐ MK-886 (classical FLAP inhibitor), fiboflapon (GSK2190915, anti-asthma FLAP inhibitor in clinical development), and emerging FLAP-targeted approaches in asthma, atherosclerosis, and emerging inflammatory disease therapeutics.

Primary applications: • Leukotriene synthesis: LTB4, cysteinyl leukotrienes (LTC4, LTD4, LTE4) quantification by LC-MS in FLAP-null cells — should be completely abolished. • FLAP inhibitor specificity: critical genetic control for ⭐ MK-886, fiboflapon (GSK2190915), and emerging FLAP-targeted compounds. • 5-LOX pathway dissection: paired analysis with ALOX5 KO in HEK293 (also available) for complete 5-LOX axis characterization. • Asthma pathology: in heterologous neutrophil/eosinophil-relevant contexts, leukotriene-mediated inflammation studies. EDITGENE recommends this FLAP KO paired with ALOX5 KO for systematic 5-LOX leukotriene pathway research and asthma drug development.

Yes. FLAP rescue experiments require attention to nuclear envelope membrane architecture: • Construct design: use a codon-modified ALOX5AP sequence with a small intracellular tag — FLAP is a small (~18 kDa) integral membrane protein with multiple transmembrane domains; small tags preserve membrane topology. • Nuclear envelope localization: confirm nuclear envelope/perinuclear localization by appropriate markers. • AA-binding-deficient rescue: AA-binding-pocket mutations affect 5-LOX activation. • Functional readout: rescue should restore A23187-induced leukotriene synthesis in 5-LOX-expressing cells. HEK293 transduces efficiently with lentivirus and supports stable rescue line generation.