ABCC1 Knockout Cell Line (HEK293)

The choice depends on whether you are studying ABCC1 (MRP1, multidrug resistance protein 1)'s role as a multidrug efflux transporter or modeling its functions in drug resistance and physiological transport. The Knockout line is the standard tool for asking whether ABCC1/MRP1 is required for these processes — ABCC1/MRP1 is an ATP-binding cassette transporter that effluxes glutathione conjugates, glucuronides, sulfates, and a broad range of chemotherapeutics (doxorubicin, vincristine, etoposide, methotrexate); MRP1 is one of the three major drug-efflux ABC transporters (with ABCB1/P-gp and ABCG2/BCRP) and a key mediator of multidrug resistance in cancer. Overexpression is useful for studying ABCC1 gain-of-function effects. For multidrug resistance research, the EDITGENE ABCC1 Knockout in HEK293 is uniquely valuable — this product completes EDITGENE's three-major-ABC-efflux-transporter toolkit alongside ABCB1/P-gp and ABCG2/BCRP (all in HEK293). Rescue with wild-type or transport-deficient ABCC1 enables structure-function studies. The knockout is a critical specificity tool for studying MRP1-mediated chemotherapy resistance, MRP1 inhibitors (MK-571, reversan), glutathione-conjugate transport, and emerging MRP1-targeted approaches in drug resistance.

Primary applications: • Multidrug efflux: chemotherapeutic (doxorubicin, vincristine, etoposide) efflux and accumulation analysis in MRP1-null cells. • Glutathione conjugate transport: GSH conjugate (LTC4, GSH-drug conjugates) transport analysis. • MRP1 inhibitor specificity: critical genetic control for MK-571, reversan, and emerging MRP1 inhibitors. • Three-transporter MDR toolkit: combined analysis with ABCB1/P-gp and ABCG2/BCRP KOs in HEK293 (all available) for systematic multidrug resistance studies. EDITGENE recommends this model as part of the complete three-major-ABC-efflux-transporter toolkit for multidrug resistance research.

Yes. ABCC1/MRP1 rescue experiments are well-established for drug transporter research: • Construct design: use a codon-modified ABCC1 sequence with a small intracellular tag (FLAG, HA). MRP1 is a full ABC transporter with three membrane-spanning domains and two nucleotide-binding domains — preserve membrane topology. • Surface localization validation: confirm plasma membrane localization before efflux assays. • Transport-deficient rescue: Walker A/B motif mutations abolish ATP-dependent transport. • Functional readout: rescue should restore substrate efflux measured by calcein-AM or doxorubicin accumulation assays. HEK293 transduces efficiently with lentivirus and supports stable rescue line generation.