ABCB1 Knockout HEK293 Cell Line

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ABCB1 Knockout HEK293 Cell Line
Cat.No.:

EDC07523

Species:

Human

Cell Name:

HEK293

Gene:

ABCB1

Gene ID:

5243

Size:

1×10⁶cells

ABCB1 Knockout Cell Line (HEK293) is an exclusive upgraded CRISPR/Cas9 system-mediated gene knockout cell, with the advantages of Optimized Strategy Design, Efficient Cell Transfection, High-Performance Cas9 Protein and Hassle-Free Cell Selection.
Cat.No. EDC07523
Product Name ABCB1 Knockout Cell Line (HEK293)
Cell Line HEK293
Cellosaurus ID CVCL_0045
Cell Line Synonyms Hek293, HEK-293, HEK/293, (HEK)293, HEK 293, HEK,293, 293, 293 HEK, 293 Ad5, Graham 293, Graham-293, Human Embryonic Kidney 293
Gene ABCB1
NCBI Gene ID
Gene Synonyms ABC20|CD243|CLCS|ENPAT|GP170|MDR1|P-GP|PGY1|p-170
Summary
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Feb 2017]
Associated Diseases Non-tumor
Morphology Adherent
Passage Ratio 1/5，2days
Complete Culture Medium DMEM + 10% FBS
Freezing Medium 95% Complete culture medium+ 5% DMSO
QC Indels validated by Sanger sequencing; sterility confirmed via microbial testing.
* For research use only. Not intended for use in humans or animals, including clinical, therapeutic, or diagnostic purposes.
LociSTR Info (Sample Cell)
Sample Cell Line: HEK293		STR Info (Cell bank)
Cell Line: HEK293
Allele1Allele2Allele1Allele2
Amelogenin X X
CSF1P0 12 11 12
D2S1338 19 19
D3S1358 15 17 15 17
D5S818 8 8 9
D7S820 11 12 11 12
D8S1179 12 14 12 14
D13S317 12 14 12 14
D16S539 9 13 9 13
D18S51 17 18 17 18
D19S433 15 18 15 18
D21S11 28 30.2 28 30.2
FGA 23 23
Penta D 9 10 9 10
Penta E 7 15 7 15
TH01 7 9.3 7 9.3
TPOX 11 11
vWA 16 19 16 19
D6S1043 11 11
D12S391 19 21 11 15
D2S441 11 15 11 15
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

FAQ

The choice depends on whether you are studying ABCB1 (P-glycoprotein, P-gp, MDR1)'s role as the prototypical multidrug efflux transporter or modeling its central functions in drug resistance and pharmacokinetics. The Knockout line is the standard tool for asking whether ABCB1/P-gp is required for these processes — ABCB1/P-gp is the founding member of the ABC drug-efflux transporters, a full-transporter that effluxes an extraordinarily broad range of hydrophobic substrates including chemotherapeutics (doxorubicin, paclitaxel, vinblastine, imatinib), HIV protease inhibitors, immunosuppressants, and CNS drugs; P-gp is highly expressed at the blood-brain barrier, intestine, liver, and kidney, fundamentally shaping drug pharmacokinetics, and is the classical mediator of cancer multidrug resistance. Overexpression is useful for studying ABCB1 gain-of-function effects. For multidrug resistance and pharmacokinetics research, the EDITGENE ABCB1 Knockout in HEK293 is uniquely valuable — P-gp is the single most studied multidrug resistance transporter. Rescue with wild-type or transport-deficient ABCB1 enables structure-function studies. The knockout is a critical specificity tool for studying P-gp-mediated chemotherapy resistance, P-gp inhibitors (verapamil, tariquidar, elacridar, valspodar), blood-brain barrier drug penetration (P-gp is a major obstacle to CNS drug delivery), and drug-drug interaction pharmacokinetics — P-gp is a required transporter in FDA/EMA drug-drug interaction guidance.
Primary applications: • P-gp-mediated efflux: chemotherapeutic (doxorubicin, paclitaxel, rhodamine 123, calcein-AM) efflux and accumulation analysis in P-gp-null cells. • Multidrug resistance: chemotherapy resistance analysis given P-gp's central MDR role. • P-gp inhibitor specificity: critical genetic control for verapamil, tariquidar, elacridar, valspodar. • Blood-brain barrier penetration: in heterologous BBB-relevant contexts, P-gp-mediated CNS drug efflux studies (a major obstacle to CNS drug delivery). • Drug-drug interaction: P-gp substrate/inhibitor characterization (required in FDA/EMA DDI guidance). EDITGENE recommends this model as the cornerstone of the complete ABC-efflux-transporter toolkit for multidrug resistance and pharmacokinetics research.
Yes. ABCB1/P-gp rescue experiments are well-established for drug transporter research: • Construct design: use a codon-modified ABCB1 sequence with a small intracellular tag (FLAG, HA). P-gp is a full ABC transporter with two transmembrane domains and two nucleotide-binding domains — preserve membrane topology and the broad substrate-binding pocket. • Surface localization validation: confirm plasma membrane localization by cell surface staining (UIC2 antibody) before efflux assays. • Transport-deficient rescue: K433M/K1076M Walker A mutations abolish ATP-dependent transport. • Functional readout: rescue should restore substrate efflux measured by rhodamine 123/calcein-AM accumulation assays. HEK293 transduces efficiently with lentivirus and supports stable rescue line generation.
* Research Use Disclaimer: Content is generated from publicly available research data, bioinformatic resources, and computational analyses for research reference only.

Related Publications

IF=3.7
Insect biochemistry and molecular biology
ATP binding cassette (ABC) transporters are a diverse family of transmembrane proteins. Specific subfamily members expressed in the lepidopteran midgut can act as susceptibility determinants for several insecticidal Bt Cry proteins. However, the susceptibility determinants to many Cry toxins still remain unclear. Therefore, we knocked out a series of ABC transporters that are highly expressed in the midgut of Bombyx mori larvae by transcription activator-like effector nuclease (TALEN)-mediated gene editing, and the lineages that became resistant to Cry toxins were searched by toxin overlay bioassay. As a result, the B. mori ABC transporter subfamily B1 (BmABCB1) knockout lineage showed 19.17-fold resistance to Cry1Ba, 876.2-fold resistance to Cry1Ia, and 29.1-fold resistance to Cry9Da, suggesting that BmABCB1 is the determinant of susceptibility to these toxins. BmABCC2 and BmABCC3 have been shown to be susceptibility determinants based on their function as receptors. Therefore, we next heterologously expressed these ABC transporters in HEK293T cells and performed a cell swelling assay to examine whether these molecules could exert receptor functions. As a result, BmABCB1-expressing cells showed swelling response to Cry1Ia and Cry9Da, and cells expressing PxABCB1, which is the Plutella xylostella ortholog of BmABCB1, showed swelling for Cry1Ba, suggesting that ABCB1 is a susceptibility determinant by functioning as a receptor to these toxins. Furthermore, in order to clarify how high binding affinity is based on receptor function, we performed surface plasmon resonance analysis and found that each KD of Cry1Ba, Cry1Ia, and Cry9Da to BmABCB1 were 7.69 × 10 M, 2.19 × 10 M, and 4.17 × 10 M respectively.
This KO model may be useful for: - Functional validation of ABCB1 as a receptor for Cry1Ba, Cry1Ia, and Cry9Da toxins in insecticidal Bt protein susceptibility - Cell swelling assays to assess receptor-mediated toxin responses in heterologous expression systems - Surface plasmon resonance analysis to quantify binding affinities (KD) between Cry toxins and ABCB1 - Comparative ortholog studies (e.g., BmABCB1 vs. PxABCB1) to evaluate cross-species toxin-receptor interactions - Toxin overlay bioassays to screen for resistance mechanisms against specific Bt Cry proteins

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