Single-cell editing platform
Traditional cell line generation is a grueling process: polyclonal pool screening → positive monoclonal screening → monoclonal expansion. This typically takes 10-14 weeks and suffers from dominant clone bias, which compromises the representativeness of the final cell line.
EDITGENE has developed a disruptive Single-Cell Gene Editing Platform that deeply integrates single-cell dispensing, single-cell culturing, and gene editing. Utilizing the cutting-edge German Cytena UP.SIGHT system, single cells are precisely deposited into 96-well plates. Combined with our proprietary CFM cloning supplement (which drastically boosts cloning efficiency) and Flash-KO in-situ editing, researchers can obtain single-cell-derived edited lines in one step. This completely bypasses traditional polyclonal screening and shrinks the turnaround time to just 6-8 weeks.
UP.SIGHT Single-Cell Dispensing
CFM Cloning Supplement
FLASH Delivery Method
Rapid Cycle
| Comparison Metric | Conventional Limiting Dilution | Electroporation / Viral Transfection + Selection |
|---|---|---|
| Monoclonal Isolation Method | Manual limiting dilution; high randomness; prone to polyclonal contamination | Limiting dilution after polyclonal pool screening; cumbersome steps |
| Multi-round Screening | Yes (2-3 rounds) | Yes (Antibiotic selection + Limiting dilution) |
| Turnaround Time | 12-20 Weeks | 14-18 Weeks |
| Cloning Efficiency | 10-30% (Cell-line dependent) | 10-30% |
| KO Success Rate (Monoclonal) | 30-50% | 40-60% |
| Cellular Damage | Low (but requires frequent passaging) | High (Electroporation and selection stress) |
| Automation Level | Manual; high batch-to-batch variance | Semi-automated |
| Applicable Cell Types | Standard adherent cells | Broad |
| Single-Cell Gene Editing Platform |
|---|
| UP.SIGHT precision plating; >99% single-cell accuracy |
| No: One-step monoclonal acquisition |
| 8-10 Weeks (30% reduction) |
| 31-62% (62.5% for 293T, 31.25% for HeLa) |
| 60-100% (100% for HeLa, 80% for 293T) |
| Low (In situ transfection; no trypsinization required) |
| Fully Automated (UP.SIGHT image recognition & dispensing) |
| Broad (Covers both adherent and suspension cells) |
Single Gene KO (Standard)
8-10 Weeks- UP.SIGHT Plating + CFM Supplement + FLASH-RNP
- KO in standard adherent/suspension cell lines
- Homozygous monoclonal lines (≥2) + Sequencing report + Colony images
Multi-gene Co-knockout
10-12 Weeks- Simultaneous delivery of multiple sgRNAs + Single-cell screening
- Double/Triple gene combined KO (e.g., immune checkpoint combinations)
- Homozygous multi-KO monoclonal lines + Validation report
High-Throughput Arrayed KO
12-16 Weeks- 96-well pre-designed sgRNA library + Automated plating/transfection
- Genome-wide screening, drug target discovery, pathway analysis
- Monoclonal cell line per well (pool or clone options)
Point Mutation (Custom)
12-16 Weeks- Single-cell plating + FLASH-PE7 Prime Editing
- SNVs, small indels (e.g., disease models)
- Homozygous PM monoclonal lines + Sequencing report
Gene Knock-in (Custom)
12-16 Weeks- Single-cell plating + FLASH-KI (RNP+Donor) + NHEJ Inhibitor
- Reporter KI, tag insertion, conditional alleles
- Homozygous KI monoclonal lines + Sequencing/Flow report
293T B2M Knockout: Efficient Acquisition of 48 Homozygous Clones
Objective: Construct B2M knockout cell lines in 293T cells.
Workflow: UP.SIGHT single-cell plating → CFM-promoted cloning → FLASH-KO transfection.
Results: Achieving a 62.5% colony formation rate in 96-well plates and an 80% success rate for homozygous KO monoclones. The project cycle was shortened by 2-3 weeks.
A549 & HeLa B2M Knockout: 100% Knockout Success Rate
Objective: Construct B2M knockout cell lines in A549 and HeLa cells.
Workflow: UP.SIGHT single-cell plating → CFM-promoted cloning → FLASH-KO transfection.
Results: Formed 38 and 65 clones in 96-well plates respectively, with a 100% success rate for homozygous KO monoclones. High-quality cell lines were obtained in one step without additional screening.
HAP1 High-Throughput Knockout of 96 Different Genes
Objective: Simultaneously construct knockout lines for 96 distinct genes in HAP1 cells.
Workflow: 96-well single-cell plating → Target-specific FLASH-KO transfection per well.
Results: 70 clones were formed (72.9% efficiency), yielding 42 homozygous KO monoclones (60% success rate). This demonstrates the platform’s high reliability for high-throughput gene editing.
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