Gene Delivery Platform
The methods of introducing exogenous nucleic acids into cells can be divided into chemical transfection, physical transfection, and biological transfection according to different delivery methods. Specifically, chemical transfection involves the use of liposomes and cationic polymer PEI; Physical transfection includes electroporation and microinjection; And biological transfection involves lentivirus packaging transfection, etc.
EDITGENE focuses on providing high-quality gene editing services and has established a mature gene delivery platform, including lentivirus transfection system, electroporation experimental system, liposome transfection, as well as Cas9 editing system based on small molecule RNP delivery. By summarizing thousands of CRO projects internally, we are able to provide an efficient delivery system according to experimental requirements for solving your experimental problems, such as low transfection efficiency of difficult cells and poor cell status after transfection for accelerating your research progress.
Advantages
Mature electroporation experimental system
Mature RNP transfection system
Exclusive optimized lentivirus packaging system
Multiple vector backbones options
Related technical services and products
Data Case
The high efficient transfection reagent CRISPR EDITx™Tran independently developed by EDITGENE can deliver the RNP gene editing system to various cell types with detectable extremely high editing efficiency.
Cell type Cell type | Cell source | Knockout efficiency |
---|---|---|
THP-1 | Human monocytic leukemia cell line | ![]() ![]() |
Hela | Human cervical cancer cell line | ![]() ![]() |
A549 | Human non-small cell lung cancer line | ![]() ![]() |
C2C12 | Mouse myoblast cell line | ![]() ![]() |
MDA-MB-231 | Human breast cancer cell line | ![]() ![]() |
SNU-449 | Human liver cancer cell line | ![]() ![]() |
H1975 | Human liver cancer cell line | ![]() ![]() |
*Knockout efficiency (%): ★★★★> 81%, ★★★ 51-80%
The above cell lines are using the high efficient transfection reagent CRISPR EDITx™Tran that independently developed by EDITGENE for RNP transfection
The EDITGENE deliverd the HES-KI system into K562 cells by electroporation, achieving the successful insertion and expression of EGFP at the Safety Harbor site.
1)Method
2)Result
Cell Pool without pressure screening with HES-KI System by electroporation in K562 Cells
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