Gene Delivery Platform

The methods of introducing exogenous nucleic acids into cells can be divided into chemical transfection, physical transfection, and biological transfection according to different delivery methods. Specifically, chemical transfection involves the use of liposomes and cationic polymer PEI; Physical transfection includes electroporation and microinjection; And biological transfection involves lentivirus packaging transfection, etc.

EDITGENE focuses on providing high-quality gene editing services and has established a mature gene delivery platform, including lentivirus transfection system, electroporation experimental system, liposome transfection, as well as Cas9 editing system based on small molecule RNP delivery. By summarizing thousands of CRO projects internally, we are able to provide an efficient delivery system according to experimental requirements for solving your experimental problems, such as low transfection efficiency of difficult cells and poor cell status after transfection for accelerating your research progress.

Advantages

Mature electroporation experimental system
equipped with specialized instruments and over 200 electroporation protocols, with a delivery efficiency of up to 80%
Mature RNP transfection system
Exclusive developed CRISPR EDITx Tran RNP transfection reagent, cytotoxic free, with extremely high delivery efficiency
Exclusive optimized lentivirus packaging system
Optimized packaging plasmids and equipped with efficient transfection reagents. Compatible with multiple plasmid systems, with short packaging cycles and high virus titers
Multiple vector backbones options
With a rich list of vector backbone, suitable vectors can be selected according to experimental requirements for saving time in vector construction

Related technical services and products

Data Case

1. CRISPR EDITx ™ Tran transfection reagent
The high efficient transfection reagent CRISPR EDITx™Tran independently developed by EDITGENE can deliver the RNP gene editing system to various cell types with detectable extremely high editing efficiency.
 Cell type Cell type Cell source Knockout efficiency
THP-1 Human monocytic leukemia cell line
Hela Human cervical cancer cell line
A549 Human non-small cell lung cancer line
C2C12 Mouse myoblast cell line
 MDA-MB-231 Human breast cancer cell line
SNU-449 Human liver cancer cell line
H1975 Human liver cancer cell line

*Knockout efficiency (%): ★★★★> 81%, ★★★ 51-80%

The above cell lines are using the high efficient transfection reagent CRISPR EDITx™Tran that independently developed by EDITGENE for RNP transfection



2. Mature electroporation system 
The EDITGENE deliverd the HES-KI system into K562 cells by electroporation, achieving the successful insertion and expression of EGFP at the Safety Harbor site.

       1)Method


       2)Result



Cell Pool without pressure screening with HES-KI System by electroporation in K562 Cells

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