Details Product Information
| Product Name | nuNPG Severe Combined Immunodeficient Mouse |
|---|---|
| Strain Name | NOD-Prkdctm1Ming-IL2rgtm1Ming/MC |
| Advantages |
1. The most severely immunodeficient mouse model currently available. 2. No residual Prkdc expression due to complete deletion of exons 2–10. 3. Minimal rejection of human cells and tissues. |
| Application |
1. Human cell or tissue transplantation 2. Cancer and cancer stem cell research 3. ES and iPS cell research Hematopoietic and immunological studies 4. Infectious disease models of human pathogens 5. Development of new humanized mouse m |
Modeling Principle
The nuNPG strain is a severe immunodeficient mouse model developed on an inbred NOD genetic background. It carries two engineered mutations: Prkdctm1Ming and IL2rgtm1Ming.
The Prkdctm1Ming mutation is a knockout (KO) achieved by deleting exons 2–10 of the Prkdc gene. This disruption blocks the development of T and B cells, leading to immunodeficiency. Unlike the spontaneous PrkdcSCID mutation in SCID mice, Prkdctm1Ming does not exhibit the age-related leaky expression of Prkdc, thus avoiding partial immune recovery.
The IL2rgtm1Ming mutation is a KO of exons 3–6 of the IL2rg gene, which encodes the common gamma chain (γc) shared by multiple interleukin receptors, including IL-2, IL-4, IL-7, IL-9, and IL-15. This gene is essential for the differentiation and function of various hematopoietic cells and is critical for natural killer (NK) cell development.
On the NOD background, the combination of Prkdctm1Ming and IL2rgtm1Ming mutations results in a profound immunodeficiency characterized by a complete lack of T, B, and NK lymphocyte lineages.
